Label-free CRP detection using optical biosensor with one-step immobilization of antibody on nitrocellulose membrane

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Title
Label-free CRP detection using optical biosensor with one-step immobilization of antibody on nitrocellulose membrane
Author(s)
Bobae Kim; W J Im; Ju Young Byun; H M Kim; M G Kim; Yong Beom Shin
Bibliographic Citation
Sensors and Actuators B-Chemical, vol. 190, pp. 243-248
Publication Year
2014
Abstract
To assess the potential of using the MCLW sensor as a label-free optical biosensor system for clinical diagnosis, we evaluated the potential of detecting C-reactive protein (CRP) in a diluted real serum sample by using nitrocellulose as an adlayer. Nitrocellulose was coated on the MCLW sensor chip via spin coating and the CRP antibody was directly immobilized on the sensor surface by hydrophobic physical adsorption. Biosensing using the nitrocellulose surface is highly convenient and does not require any additional treatment for the immobilization of ligand. We optimized the condition for the deposition of nitrocellulose on the MCLW sensor chip with the goal of generating the largest signal difference of the sample against the control signal. In addition, label-free immunosensing of CRP was achieved using the nitrocellulose-coated MCLW sensor in real time. As a result, we were able to quantitatively detect CRP in a human serum within a concentration range of 0.1-10 μg/mL.
Keyword
C-reactive proteinLabel-freeMetal clad waveguideNitrocelluloseOptical biosensor
ISSN
0925-4005
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.snb.2013.08.078
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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