Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration

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Title
Establishment of a transgenic zebrafish EF1α:Kaede for monitoring cell proliferation during regeneration
Author(s)
H Y Moon; O H Kim; H T Kim; J H Choi; S Y Yeo; Nam-Soon KimDoo-Sang ParkHyun Woo Oh; K H You; M D Zoysa; C H Kim
Bibliographic Citation
Fish & Shellfish Immunology, vol. 34, no. 5, pp. 1390-1394
Publication Year
2013
Abstract
Zebrafish is considered as a versatile experimental animal for various research models from development to diseases. In this study, we report the development of transgenic zebrafish line named as Tg(EF1α:Kaede) that expresses translation elongation factor 1 subunit alpha (EF1α) promoter linked to a fluorescent protein (FP), Kaede for monitoring proliferating cells in during regeneration. It was revealed that about 1.4 kb 5'-flanking region of the EF1α was sufficient for its promoter activity. Expression of Kaede with a property of photo-conversion from green to red was detected in different embryonic stages as well as various organs such as brain, heart, pancreas, intestine, ovary, and fins of adult fish. Cell proliferation pattern during fin regeneration was monitored after amputation of Tg(EF1α:Kaede) caudal fin and results shown that this system is simple and efficient method for detecting proliferating cells during tissue regeneration. Developed Tg(EF1α:Kaede) line has potential to investigate the cell proliferation, regeneration, wound healing capacities after tissue damage and evaluate the therapeutic power of wound healing drugs.
Keyword
Cell proliferationKaedeRegenerationTranslation elongation factor 1Zebrafish
ISSN
1050-4648
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.fsi.2013.02.021
Type
Article
Appears in Collections:
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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