In vitro and in vivo assessment of cytochrome P450-mediated herb-drug interaction of Ssang-hwa-tang

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Title
In vitro and in vivo assessment of cytochrome P450-mediated herb-drug interaction of Ssang-hwa-tang
Author(s)
S Y Lee; J Y Lee; W Kang; K I Kwon; Soo Jin Oh; J Y Ma; S K Kim
Bibliographic Citation
Food Chemistry, vol. 136, no. 2, pp. 450-457
Publication Year
2013
Abstract
We have evaluated the herb-drug interaction potential of Ssang-hwa-tang (SHT) mediated by cytochrome P450 (CYP) inhibition/induction. Further, the effects of fermentation on the CYP-mediated herb-drug interaction potential were determined. SHT showed inhibitory activity toward CYP1A2, but not 2A6, 2B6, 2C9, 2C19, 2D6, 2E1, and 3A4 in human liver microsomes. The results of the enzyme kinetic study suggested that the SHT-induced CYP1A2 inhibition is mixed reversible inhibition. The hepatic CYP expression and activity in rats treated with SHT were examined. The expression/activity of CYP2E1 increased as a result of SHT extract treatment (P < 0.005 or P < 0.001, respectively), which raises the possibility that SHT may increase the toxicity of environmental toxicants through the elevation of CYP2E1-mediated metabolic activation. SHT fermentation using Lactobacillus fermentum or Lactobacillus gasseri resulted in attenuation of the SHT-induced CYP1A2 inhibition, but not CYP2E1 induction, suggesting that changes in the chemical composition of SHT through fermentation can affect the inhibition of CYP1A2 activity.
Keyword
Cytochrome P450FermentationHerb-drug interactionSsang-hwa-tang
ISSN
0308-8146
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.foodchem.2012.08.069
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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