DC Field | Value | Language |
---|---|---|
dc.contributor.author | K Nakahira | - |
dc.contributor.author | A Morita | - |
dc.contributor.author | Nam-Soon Kim | - |
dc.contributor.author | I Yanagihara | - |
dc.date.accessioned | 2017-04-19T09:44:17Z | - |
dc.date.available | 2017-04-19T09:44:17Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | 10.1371/journal.pone.0077099 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/11573 | - |
dc.description.abstract | Forkhead Box P3 (FOXP3) is a member of the forkhead/winged helix family of the transcription factors and plays an important role not only as a master gene in T-regulatory cells, but also as a tumor suppressor. In this study, we identified lymphocyte-specific protein tyrosine kinase (LCK), which correlates with cancer malignancy, as a binding partner of FOXP3. FOXP3 downregulated LCK-induced MMP9, SKP2, and VEGF-A expression. We observed that LCK phosphorylated Tyr-342 of FOXP3 by immunoprecipitation and in vitro kinase assay, and the replacement of Tyr-342 with phenylalanine (Y342F) abolished the ability to suppress MMP9 expression. Although FOXP3 decreased the invasive ability induced by LCK in MCF-7 cells, Y342F mutation in FOXP3 diminished this suppressive effect. Thus we demonstrate for the first time that LCK upregulates FOXP3 by tyrosine phosphorylation, resulting in decreased MMP9, SKP2, and VEGF-A expression, and suppressed cellular invasion. We consider that further clarification of transcriptional mechanism of FOXP3 may facilitate the development of novel therapeutic approaches to suppress cancer malignancy. | - |
dc.publisher | Public Library of Science | - |
dc.title | Phosphorylation of FOXP3 by LCK downregulates MMP9 expression and represses cell invasion = FOXP3의 인산화에 의한 MMP의 발현 및 세포 전이 억제 | - |
dc.title.alternative | Phosphorylation of FOXP3 by LCK downregulates MMP9 expression and represses cell invasion | - |
dc.type | Article | - |
dc.citation.title | PLoS One | - |
dc.citation.number | 10 | - |
dc.citation.endPage | e77099 | - |
dc.citation.startPage | e77099 | - |
dc.citation.volume | 8 | - |
dc.contributor.affiliatedAuthor | Nam-Soon Kim | - |
dc.contributor.alternativeName | Nakahira | - |
dc.contributor.alternativeName | Morita | - |
dc.contributor.alternativeName | 김남순 | - |
dc.contributor.alternativeName | Yanagihara | - |
dc.identifier.bibliographicCitation | PLoS One, vol. 8, no. 10, pp. e77099-e77099 | - |
dc.identifier.doi | 10.1371/journal.pone.0077099 | - |
dc.description.journalClass | Y | - |
Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.