Carbohydrate derivatives from the roots of Brassica rapa ssp. campestris and their effects on ROS production and glutamate-induced cell death in HT-22 cells

Cited 22 time in scopus
Metadata Downloads
Title
Carbohydrate derivatives from the roots of Brassica rapa ssp. campestris and their effects on ROS production and glutamate-induced cell death in HT-22 cells
Author(s)
Q Wu; J G Cho; D S Lee; D Y Lee; N Y Song; Y C Kim; K T Lee; H G Chung; M S Choi; Tae Sook Jeong; E M Ahn; G S Kim; N I Baek
Bibliographic Citation
Carbohydrate Research, vol. 372, pp. 9-14
Publication Year
2013
Abstract
Phytochemical investigation of the roots of Brassica rapa ssp. campestris led to the isolation of three new carbohydrate derivatives, namely sucrose 3,3′,4′-triisovalerate (2), sucrose 6,3′,4′- triisovalerate (3), and ethanone-1-C-β-d-glucopyranoside (3,7-anhydro-1-deoxy-d-glycero-d-gulo-2-octulose, 6), along with four known carbohydrate derivatives, 2,6,3′,4′-tetraisovalerate (1), ethyl β-d-glucopyranoside (4), n-butyl β-d-fructofuranoside (5), and n-pentyl β-d-fructofuranoside (7), which were initially isolated from plants of the Brassica genus. Structures of the isolated compounds were established by spectroscopic analyses, including UV, IR, MS, and NMR. All of the isolated carbohydrate derivatives were evaluated to determine their effect on ROS production and glutamate-induced cell death in HT-22 cells. Compound 6 showed the most significant ROS reduction and a protective effect with IC 50 values of 69.4 ± 3.8 μM and 4.96 ± 0.32 μM, respectively, which were equivalent to those of the positive control, Trolox.
Keyword
3,7-Anhydro-1-deoxy-d-glycero-d-gulo-2-octuloseBrassica rapa ssp. campestrisEthanone-1-C-β-d- glucopyranosideReactive oxygen speciesSucrose 3,3′,4′-triisovalerateSucrose 6,3′,4′-triisovalerate
ISSN
0008-6215
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.carres.2012.09.015
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.