Arazyme inhibits cytokine expression and upregulates skin barrier protein expression

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Title
Arazyme inhibits cytokine expression and upregulates skin barrier protein expression
Author(s)
I S Kim; M J Kim; D H Shin; Kwang-Hee SonHo Yong Park; J S Lee
Bibliographic Citation
Molecular Medicine Reports, vol. 8, no. 2, pp. 551-556
Publication Year
2013
Abstract
In the present study, the inhibitory effect of arazyme on allergic inflammation was investigated by evaluating the alteration of cytokine production and expression of skin barrier proteins in immune and HaCaT human keratinocyte cells. THP-1 human monocytic and EoL-1 human eosinophilic cells were treated with Dermatophagoides pteronissinus extract (DpE). Monocyte chemotactic protein-1 (MCP-1)/CCL2, interleukin (IL)-6 and IL-8 increased following DpE treatment and arazyme significantly blocked the increase of MCP-1, IL-6 and IL-8 expression in cell types. Secretion of MCP-1, IL-6 and IL-8 induced by lipopolysaccharide in THP-1 cells was also inhibited by arazyme treatment. Arazyme inhibited the secretion of IL-6 and IL-8 due to phorbol 12-myristate 13-acetate and calcium ionophores in human mast cells. Arazyme blocked the secretion of thymus and activation-regulated chemokine (TARC)/CCL17, MCP-1, IL-6 and IL-8 due to tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in HaCaT cells. TNF-α and IFN-γ suppressed the expression of skin barrier proteins, including filaggrin, involucrin and loricrin. By contrast, arazyme increased the expression of filaggrin, involucrin and loricrin. These results may contribute to the development of a therapeutic drug for the treatment of allergic diseases, including atopic dermatitis.
Keyword
Allergic inflammationArazymeCytokineSkin barrier protein
ISSN
1791-2997
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/mmr.2013.1520
Type
Article
Appears in Collections:
Division of Biomaterials Research > Industrial Bio-materials Research Center > 1. Journal Articles
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