Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi = RNAi 상태의 SMV-CP 유전자의 유전적 형질전환에 의해 생산된 콩의 SMV 저항성의 특성분석

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Title
Characterization of SMV resistance of soybean produced by genetic transformation of SMV-CP gene in RNAi = RNAi 상태의 SMV-CP 유전자의 유전적 형질전환에 의해 생산된 콩의 SMV 저항성의 특성분석
Author(s)
H J Kim; M J Kim; J H Pak; H W Jung; H K Choi; Y H Lee; I Y Baek; J M Ko; Soon Chun Jeong; In Soon Pack; K H Ryu; Y S Chung
Bibliographic Citation
Plant Biotechnology Reports, vol. 7, no. 4, pp. 425-433
Publication Year
2013
Abstract
Soybean mosaic virus (SMV), a species of the Potyvirus genus in the Potyviridae family, is one of the most typical viral diseases and results in yield and quality loss of cultivated soybean. Due to the depletion of genetic resources for resistance breeding, a trial of genetic transformation to improve disease resistance has been performed by introducing the SMV-CP gene by the RNA interference (RNAi) method via Agrobacterium-mediated transformation. Among 30 transgenic plants produced, 7 lines with enough seeds were infected with SMV and two lines (3 and 4) showed viral resistance to SMV infection. In genomic Southern blot analysis, all the lines tested contained at least one T-DNA insertion. Subsequent investigation confirmed that no viral CP gene expression was detected in two SMV-resistant lines after artificial inoculation of SMV, while non-transgenic control and other transgenic lines expressed substantial amounts of the viral gene. Viral symptoms affected seed morphology, and clean seeds were harvested from the resistant lines. Also, strong viral gene expression was detected from the seeds of susceptible lines. In further generations, the same phenotypic appearance was maintained among non-transgenic and transgenic plants. Finally, the presence of helper component-proteinase (HC-Pro), known as a suppressor of gene silencing apparatus, was checked among transgenic lines. No expression of HC-Pro in resistant lines indicated that the viral CP-RNAi transformation into soybean somehow created a functional gene silencing system and resulted in a viral-resistant phenotype.
Keyword
Agrobacterium-mediated transformationCoat proteinHelper component proteinase (HC-Pro)RNA interferenceSoybean mosaic virus (SMV)
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-013-0279-y
Type
Article
Appears in Collections:
Ochang Branch Institute > 1. Journal Articles
Division of Bio Technology Innovation > Bio-Evaluation Center > 1. Journal Articles
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