High-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis = 약모밀의 접합자배 배양을 통한 식물체 재생체계 확립

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Title
High-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis = 약모밀의 접합자배 배양을 통한 식물체 재생체계 확립
Author(s)
M J Oh; Myung-Suk Ahn; Eun-Yee Jie; Jang Ryol Liu; B W Min; Suk Weon Kim
Bibliographic Citation
Plant Biotechnology Reports, vol. 7, no. 4, pp. 527-534
Publication Year
2013
Abstract
This study reports high-frequency plant regeneration from immature zygotic embryo cultures of Houttuynia cordata Thunb via somatic embryogenesis. Numerous green globular structures were directly formed on the surfaces of cotyledons and radicles from 2-week-old immature zygotic embryos at a frequency of 42.1 % when cultured on Murashige and Skoog (MS) medium supplemented with 2 mg l-1 of α-naphthaleneacetic acid (NAA) and 1 mg l-1 of 6-benzyladenine (BA). In comparison, white globular structures and pale-yellow calluses were formed simultaneously at a frequency of 28.3 % when cultured on MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D). The pale-yellow calluses were transferred to MS liquid medium supplemented with 2,4-D to establish embryogenic cell suspension cultures consisting of round, isodiametric cells that formed cell aggregates. Upon plating of these cell aggregates on half-strength MS medium without growth regulators under light conditions, cell aggregates gave rise to numerous globular embryos at a frequency of 56 %. Of the globular embryos, 15 % were successfully converted into cotyledonary embryos when cultured on half-strength MS medium under light conditions. The plant regeneration system of H. cordata established in this study will be useful for the selection, genetic transformation, and mass proliferation of elite clones with medicinal potential.
Keyword
Houttuynia cordataImmature zygotic embryo culturesPlant regenerationSomatic embryogenesis
ISSN
1863-5466
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11816-013-0291-2
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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