N-glycan analysis of human α1-antitrypsin produced in Chinese hamster ovary cells

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Title
N-glycan analysis of human α1-antitrypsin produced in Chinese hamster ovary cells
Author(s)
K J Lee; S M Lee; Jin Young Gil; Oh Suk Kwon; J Y Kim; S J Park; H S Chung; Doo-Byoung Oh
Bibliographic Citation
Glycoconjugate Journal, vol. 30, no. 5, pp. 537-547
Publication Year
2013
Abstract
Human alpha-1-antitrypsin (α1AT) is a glycoprotein with protease inhibitor activity protecting tissues from degradation. Patients with inherited α1AT deficiency are treated with native α1AT (nAT) purified from human plasma. In the present study, recombinant α1AT (rAT) was produced in Chinese hamster ovary (CHO) cells and their glycosylation patterns, inhibitory activity and in vivo half-life were compared with those of nAT. A peptide mapping analysis employing a deglycosylation reaction confirmed full occupancy of all three glycosylation sites and the equivalency of rAT and nAT in terms of the protein level. N-glycan profiles revealed that rAT contained 10 glycan structures ranging from bi-antennary to tetra-antennary complex-type glycans while nAT displayed six peaks comprising majorly bi-antennary glycans and a small portion of tri-antennary glycans. In addition, most of the rAT glycans were shown to have only core α(1 - 6)-fucose without terminal fucosylation, whereas only minor portions of the nAT glycans contained core or Lewis X-type fucose. As expected, all sialylated glycans of rAT were found to have α(2 - 3)-linked sialic acids, which was in sharp contrast to those of nAT, which had mostly α(2 - 6)-linked sialic acids. However, the degree of sialylation of rAT was comparable to that of nAT, which was also supported by an isoelectric focusing gel analysis. Despite the differences in the glycosylation patterns, both α1ATs showed nearly equivalent inhibitory activity in enzyme assays and serum half-lives in a pharmacokinetic experiment. These results suggest that rAT produced in CHO cells would be a good alternative to nAT derived from human plasma.
Keyword
Alpha1-antitrypsinAntennary structureChinese hamster ovary (CHO) cellsIn vivo half-lifeN-glycanSialic acid
ISSN
I000-0096
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10719-012-9453-7
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Aging Convergence Research Center > 1. Journal Articles
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