Patterns of protein expression in water-stressed wheat chloroplasts

Cited 27 time in scopus
Metadata Downloads
Title
Patterns of protein expression in water-stressed wheat chloroplasts
Author(s)
A H M Kamal; K Cho; J S Choi; Y Jin; C S Park; J S Lee; S H Woo
Bibliographic Citation
Biologia Plantarum, vol. 57, no. 2, pp. 305-312
Publication Year
2013
Abstract
The performance of control and water-stressed 10-d-old wheat seedlings was compared. During short-term water stress (irrigation was withheld for 9 d), rates of photosynthesis and transpiration, stomatal conductance, and relative water content decreased whereas the proline content increased. Chloroplast proteins were extracted from the leaves, separated by iso-electric focusing through two-dimensional electrophoresis, and stained with CBB R-250. Differentially expressed proteins were detected and analyzed with MALDI-TOF/TOF mass spectrometry. Under water stress, 9 proteins were up-regulated whereas 11 proteins were not affected. The ribulose-1,5-bisphospate carboxylase/oxygenase (Rubisco) small and large subunits, chloride carrier/channel family, and H+-ATPase were up-regulated by water stress whereas membrane-bound ATP synthase subunit b and cytochrome b6-f complex were down-regulated.
Keyword
chloride carrier/channel familyH+-ATPaseMALDI-TOF/TOF-MSTriticum aestivum
ISSN
0006-3134
Publisher
Acad Sciences Czech Republic, Inst Experimental Botany
DOI
http://dx.doi.org/10.1007/s10535-012-0290-0
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.