In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod

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Title
In vitro and in vivo growth inhibition of prostate cancer by the small molecule imiquimod
Author(s)
J H Han; J Lee; S J Jeon; E S Choi; S D Cho; Bo Yeon Kim; D J Kim; J H Park; J H Park
Bibliographic Citation
International Journal of Oncology, vol. 42, no. 6, pp. 2087-2093
Publication Year
2013
Abstract
Prostate cancer is the second leading cause of cancer death in men worldwide. In the present study, we examined in vitro and in vivo antitumor effect of the small molecule imiquimod, also known as a TLR7 agonist, against prostate cancer. Imiquimod inhibited the growth of mouse (TRAMP-C2) and human (PC-3) prostate cancer cells. Treatment with imiquimod induced cell cycle arrest at the G2/M phase in TRMPA-C2 cells, confirmed by the changes of G2/M checkpoint regulators such as reduction of cyclin B1 expression and increase of phospho-CDC2 and p21 in TRAMP-C2 cells treated with imiquimod. Flow cytometry and western blot analysis revealed that imiquimod induced direct apoptosis in TRAMP-C2 cells via a mitochondrial-dependent pathway. Intratumoral injection with imiquimod reduced significantly tumor growth and increased apoptotic cells in mice subcutaneously implanted with TRAMP-C2 cells. Our results indicate that imiquimod can be an alternative therapeutic for locally generated prostate cancer.
Keyword
ApoptosisCell cycle arrestImiquimodProstate cancer
ISSN
1019-6439
Publisher
Spandidos Publ Ltd
DOI
http://dx.doi.org/10.3892/ijo.2013.1898
Type
Article
Appears in Collections:
Ochang Branch Institute > Chemical Biology Research Center > 1. Journal Articles
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