Winogradskyella litorisediminis sp nov., isolated from coastal sediment

Abstract

Gram-staining-negative, non-flagellated, non-gliding, aerobic, rod-shaped bacterium, designated DPS-8T, was isolated from coastal sediment of Geoje island in the South Sea, South Korea, and subjected to a polyphasic study. Strain DPS-8T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain DPS-8T joined the clade comprising the type strains of Winogradskyella species with a high bootstrap resampling value of 93.5 %. Phylogenetic trees constructed using maximum-likelihood and maximum-parsimony algorithms revealed that strain DPS-8T belonged to the genus Winogradskyella. Strain DPS-8T exhibited 94.1-96.5 % 16S rRNA gene sequence similarity to the type strains of species of the genus Winogradskyella. Strain DPS-8T contained MK-6 as the predominant menaquinone and iso-C15: 1 G, iso-C15: 0, iso-C17: 0 3-OH and C16: 1ω7c and/or iso-C15: 0 2-OH as the major fatty acids. The major polar lipids of strain DPS-8T were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content of strain DPS-8T was 34.7 mol%. Differential phenotypic properties, together with its phylogenetic distinctiveness, revealed that strain DPS-8T is separate from recognized species of the genus Winogradskyella. On the basis of the data presented, strain DPS-8T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella litorisediminis sp. nov. is proposed. The type strain is DPS-8T (= KCTC 32110T = CCUG 62215T).