The Bdtf1 gene in alternaria brassicicola is important in detoxifying brassinin and maintaining virulence on brassica species

Cited 10 time in scopus
Metadata Downloads
Title
The Bdtf1 gene in alternaria brassicicola is important in detoxifying brassinin and maintaining virulence on brassica species
Author(s)
A Srivastava; I K Cho; Yangrae Cho
Bibliographic Citation
Molecular Plant-Microbe Interactions, vol. 26, no. 12, pp. 1429-1440
Publication Year
2013
Abstract
Brassinin is an antifungal compound induced in Brassica plants after microbial infection. Molecular evidence is incomplete, however, in supporting the importance of brassinin in plant resistance to pathogens. To test the importance of brassinin in plant defense, we studied the functions of the gene Bdtf1 in the necrotrophic fungus Alternaria brassicicola. Several strains of mutants of this gene were weakly virulent on Brassica species, causing lesions 70% smaller in diameter than the wild type on three Brassica species. These mutants, however, were as virulent as the wild type on Arabidopsis thaliana. They were similar to the wild type in spore germination, colony morphology, and mycelial growth in nutrient-rich media, both with and without stress-inducing chemicals. Unlike wild-type A. brassicicola, however, the mutants failed to germinate and their hyphal growth was arrested in the presence of 200 (μM brassinin. When grown in a medium containing 100 μM brassinin, wild-type mycelium entirely converted the brassinin into a nontoxic derivative, of which the precise chemical nature was not established. Mutants of the Bdtf1 gene were unable to perform this conversion. Our results support the hypothesis that the ability of A. brassicicola to detoxify brassinin is necessary for successful infection of Brassica species.
ISSN
0894-0282
Publisher
Amer Phytopathological Soc
DOI
http://dx.doi.org/10.1094/MPMI-07-13-0186-R
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.