Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice = TGEV S단백질을 발현하는 형질전환 식물체 개발 및 면역검정

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Title
Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice = TGEV S단백질을 발현하는 형질전환 식물체 개발 및 면역검정
Author(s)
Dong Joo Ahn; J W Youm; Suk Weon KimWoon Kee YoonHyoung-Chin Kim; T Y Hur; Y H Joung; Jae Heung JeonHyun Soon Kim
Bibliographic Citation
Korean Journal of Veterinary Research, vol. 53, no. 4, pp. 217-224
Publication Year
2013
Abstract
Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-S0.7) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-S0.7 gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-S0.7 protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-S0.7 antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.
Keyword
oral administrationpotato-derived antigentransmissible gastroenteritis virustransgenic plant
ISSN
1225-0198
Publisher
South Korea
DOI
http://dx.doi.org/10.14405/kjvr.2013.53.4.217
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > 1. Journal Articles
Division of Biomaterials Research > Plant Systems Engineering Research > 1. Journal Articles
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