BaeR protein from Salmonella enterica serovar Paratyphi A induces inflammatory response in murine and human cell lines

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dc.contributor.authorS J Lee-
dc.contributor.authorE G Awji-
dc.contributor.authorMyung Hee Kim-
dc.contributor.authorS C Park-
dc.date.accessioned2017-04-19T09:47:34Z-
dc.date.available2017-04-19T09:47:34Z-
dc.date.issued2013-
dc.identifier.issn1286-4579-
dc.identifier.uri10.1016/j.micinf.2013.09.001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11702-
dc.description.abstractBaeR is the response regulator of the two-component system, BaeSR, found in Escherichia coli (E. coli) and Salmonella. Several biological functions of BaeR, related to multidrug efflux and bacterial virulence, have been described. Herein, we report a putative function of BaeR during inflammatory response of the host by using BaeR protein of Salmonella enterica Paratyphi A (S. Paratyphi A) origin overexpressed in E. coli, and RAW 264.7 and THP-1 cells as invitro models. BaeR (3μg/ml) upregulated iNOS mRNA expression in both cell lines, and induced significant production of NO. Greater than ten-fold (TNF-α), 24-fold (IL-1β) and 156-fold (IL-6) increases in mRNA expression levels were observed in THP-1 cells treated with BaeR, compared to untreated controls. Furthermore, an eight-fold (IL-1β), 12-fold (IL-6) and 41-fold (TNF-α) higher protein concentrations were observed in RAW 264.7 cells stimulated with BaeR, compared to control cells. Immunoblot analysis showed BaeR-induced phosphorylation of the MAPKs (ERK 1/2, JNK and p38 MAPK) in RAW 264.7 cells. Pharmacological inhibition of the three MAPKs using specific inhibitors resulted in significant reduction of BaeR-induced NO production and iNOS mRNA expression by inhibitors of JNK and p38 MAPK. Also, all inhibitors of the MAPKs significantly attenuated BaeR-induced IL-1β, IL-6 and TNF-α at both transcript and protein levels with different degrees of inhibition. Taken together, our data suggest that BaeR is a putative inducer of inflammatory response and the MAPKs are involved in the process.-
dc.publisherElsevier-
dc.titleBaeR protein from Salmonella enterica serovar Paratyphi A induces inflammatory response in murine and human cell lines-
dc.title.alternativeBaeR protein from Salmonella enterica serovar Paratyphi A induces inflammatory response in murine and human cell lines-
dc.typeArticle-
dc.citation.titleMicrobes and Infection-
dc.citation.number13-
dc.citation.endPage957-
dc.citation.startPage951-
dc.citation.volume15-
dc.contributor.affiliatedAuthorMyung Hee Kim-
dc.contributor.alternativeName이승진-
dc.contributor.alternativeNameAwji-
dc.contributor.alternativeName김명희-
dc.contributor.alternativeName박승춘-
dc.identifier.bibliographicCitationMicrobes and Infection, vol. 15, no. 13, pp. 951-957-
dc.identifier.doi10.1016/j.micinf.2013.09.001-
dc.subject.keywordBaeR-
dc.subject.keywordCytokines-
dc.subject.keywordNitric oxide-
dc.subject.keywordRAW 264.7 cells-
dc.subject.keywordSalmonella enterica Paratyphi A-
dc.subject.keywordTHP-1-
dc.subject.localBaeR-
dc.subject.localcytokine-
dc.subject.localCytokines-
dc.subject.localCytokine-
dc.subject.localNO-
dc.subject.localnitric oxide-
dc.subject.localnitric oxide (NO)-
dc.subject.localNitric oxide-
dc.subject.localNO (Nitric oxide)-
dc.subject.localnitric oxide.-
dc.subject.localNitric oxid-
dc.subject.localNitric oxide (NO)-
dc.subject.localRAW 264·7 cells-
dc.subject.localRAW 264.7 cells-
dc.subject.localSalmonella enterica Paratyphi A-
dc.subject.localTHP-1-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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