Neuronal synapse formation induced by microglia and interleukin 10

Cited 161 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSo Hee Lim-
dc.contributor.authorE Park-
dc.contributor.authorB You-
dc.contributor.authorY Jung-
dc.contributor.authorA R Park-
dc.contributor.authorSung Goo Park-
dc.contributor.authorJae-Ran Lee-
dc.date.accessioned2017-04-19T09:47:40Z-
dc.date.available2017-04-19T09:47:40Z-
dc.date.issued2013-
dc.identifier.issn1932-6203-
dc.identifier.uri10.4238/2013.February.28.15ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11705-
dc.description.abstractRecently, it was found that microglia regulated synaptic remodeling of the developing brain, but their mechanisms have not been well understood. In this study, the action of microglia on neuronal synapse formation was investigated, and the primary target of microglial processes was discovered. When the developing microglia were applied to cultured hippocampal neurons without direct contact, the numbers of dendritic spines and excitatory and inhibitory synapses significantly increased. In order to find out the main factor for synaptic formation, the effects of cytokines released from microglia were examined. When recombinant proteins of cytokines were applied to neuronal culture media, interleukin 10 increased the numbers of dendritic spines in addition to excitatory and inhibitory synapses. Interestingly, without external stimuli, the amount of interleukin 10 released from the intact microglia appeared to be sufficient for the induction of synaptic formation. The neutralizing antibodies of interleukin 10 receptors attenuated the induction of the synaptic formation by microglia. The expression of interleukin 10 receptor was newly found in the hippocampal neurons of early developmental stage. When interleukin 10 receptors on the hippocampal neurons were knocked down with specific shRNA, the induction of synaptic formation by microglia and interleukin 10 disappeared. Pretreatment with lipopolysaccharide inhibited microglia from inducing synaptic formation, and interleukin 1β antagonized the induction of synaptic formation by interleukin 10. In conclusion, the developing microglia regulated synaptic functions and neuronal development through the interactions of the interleukin 10 released from the microglia with interleukin 10 receptors expressed on the hippocampal neurons-
dc.publisherPublic Library of Science-
dc.titleNeuronal synapse formation induced by microglia and interleukin 10-
dc.title.alternativeNeuronal synapse formation induced by microglia and interleukin 10-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number11-
dc.citation.endPagee81218-
dc.citation.startPagee81218-
dc.citation.volume8-
dc.contributor.affiliatedAuthorSo Hee Lim-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.affiliatedAuthorJae-Ran Lee-
dc.contributor.alternativeName임소희-
dc.contributor.alternativeName박은하-
dc.contributor.alternativeName유보람-
dc.contributor.alternativeName정영섭-
dc.contributor.alternativeName박아름-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName이재란-
dc.identifier.bibliographicCitationPLoS One, vol. 8, no. 11, pp. e81218-e81218-
dc.identifier.doi10.1371/journal.pone.0081218-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Biomedical Research > Rare Disease Research Center > 1. Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.