Identification of fibrinogen-induced nattokinase WRL101 from Bacillus subtilis WRL101 isolated from Doenjang

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dc.contributor.authorC S Park-
dc.contributor.authorD H Kim-
dc.contributor.authorW Y Lee-
dc.contributor.authorD O Kang-
dc.contributor.authorJae Jun Song-
dc.contributor.authorN S Choi-
dc.date.accessioned2017-04-19T09:47:42Z-
dc.date.available2017-04-19T09:47:42Z-
dc.date.issued2013-
dc.identifier.issn19960808-
dc.identifier.uri10.5897/AJMR12.041ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11706-
dc.description.abstractTo increase the fibrinolytic enzyme (nattokinase WRL101) in Bacillus subtilis WRL101, bovine fibrin or fibrinogen (1.0%, w/v) were added in the tryptic soy broth as a substrate. The fibrinolytic activity cultured in the fibrinogen-contained medium was increased. On the other hand, fibrin decreased the activity. Using the medium condition, nattokinase WRL101 (fibrinogen-induced nattokinase WRL101, FIN-WRL101) was isolated by commercial chromatographic techniques and its biochemical characteristics were investigated. The molecular weight of FIN-WRL101 was estimated to be 29 kDa. FIN-WRL101 was optimally active at pH 11.0 and 47°C. It had high degrading activity for the Aα-chain and B?-chain of human fibrinogen, but did not affect the ?-chain, indicating that it is an ?-fibrinogenase. FIN-WRL101 was completely inhibited by phenylmethylsulfonyl fluoride, indicating that it belongs to the serine protease. FIN-WRL101 exhibited high specificity for Meo-Suc-Arg-Pro-Tyr-pNA (S-2586), a synthetic chromogenic substrate for chymotrypsin. Its nucleotide and amino acid sequences were determined.-
dc.publisherAcademic Journalsko
dc.titleIdentification of fibrinogen-induced nattokinase WRL101 from Bacillus subtilis WRL101 isolated from Doenjang-
dc.title.alternativeIdentification of fibrinogen-induced nattokinase WRL101 from Bacillus subtilis WRL101 isolated from Doenjang-
dc.typeArticle-
dc.citation.titleAfrican Journal of Microbiology Research-
dc.citation.number19-
dc.citation.endPage1992-
dc.citation.startPage1983-
dc.citation.volume7-
dc.contributor.affiliatedAuthorJae Jun Song-
dc.contributor.alternativeName박창수-
dc.contributor.alternativeName김동호-
dc.contributor.alternativeName이우열-
dc.contributor.alternativeName강대욱-
dc.contributor.alternativeName송재준-
dc.contributor.alternativeName최낙식-
dc.identifier.bibliographicCitationAfrican Journal of Microbiology Research, vol. 7, no. 19, pp. 1983-1992-
dc.identifier.doi10.5897/AJMR12.041-
dc.subject.keywordBacillus subtilis-
dc.subject.keywordDoenjang-
dc.subject.keywordfibrinolytic enzyme-
dc.subject.keywordnattokinase.-
dc.subject.localBacillus subtilis-
dc.subject.localDoenjang-
dc.subject.localfibrinolytic enzyme-
dc.subject.localFibrinolytic enzyme-
dc.subject.localnattokinase.-
dc.description.journalClassN-
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Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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