Inactivation of metabolic genes causes short- and long- range dys-regulation in Escherichia coli metabolic network = 대사유전자 비활성화로 인한 대사네트워크 유전자 조절에 미치는 영향

Cited 10 time in scopus
Metadata Downloads
Title
Inactivation of metabolic genes causes short- and long- range dys-regulation in Escherichia coli metabolic network = 대사유전자 비활성화로 인한 대사네트워크 유전자 조절에 미치는 영향
Author(s)
D K Barupal; Sang Jun Lee; E D Karoly; S Adhya
Bibliographic Citation
PLoS One, vol. 8, no. 12, pp. e78360-e78360
Publication Year
2013
Abstract
The metabolic network in E. coli can be severely affected by the inactivation of metabolic genes that are required to catabolize a nutrient (D-galactose). We hypothesized that the resulting accumulation of small molecules can yield local as well as systemic effects on the metabolic network. Analysis of metabolomics data in wild-type and D-galactose non-utilizing mutants, galT, galU and galE, reveal the large metabolic differences between the wild-type and the mutants when the strains were grown in D-galactose. Network mapping suggested that the enzymatic defects affected the metabolic modules located both at short- and long-ranges from the D-galactose metabolic module. These modules suggested alterations in glutathione, energy, nucleotide and lipid metabolism and disturbed carbon to nitrogen ratio in mutant strains. The altered modules are required for normal cell growth for the wild-type strain, explaining why the cell growth is inhibited in the mutants in the presence of D-galactose. Identification of these distance-based dys-regulations would enhance the systems level understanding of metabolic networks of microorganisms having importance in biomedical and biotechnological research
ISSN
1932-6203
Publisher
Public Library of Science
DOI
http://dx.doi.org/10.1371/journal.pone.0078360
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:

Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.