Protection of NAD(P)H: Quinone oxidoreductase 1 against renal ischemia/reperfusion injury in mice

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Title
Protection of NAD(P)H: Quinone oxidoreductase 1 against renal ischemia/reperfusion injury in mice
Author(s)
Gil Tae Gang; Jung Hwan HwangYong-Hoon KimJung Ran NohKyoung Shim Kim; J Y Jeong; D E Choi; K W Lee; J Y Jung; M Shong; Chul Ho Lee
Bibliographic Citation
Free Radical Biology and Medicine, vol. 67, no. 1, pp. 139-149
Publication Year
2014
Abstract
Ischemia/reperfusion (I/R) is the most common cause of acute renal injury. I/R-induced reactive oxygen species (ROS) are thought to be a major factor in the development of acute renal injury by promoting the initial tubular damage. NAD(P)H:quinone oxidoreductase 1 (NQO1) is a well-known antioxidant protein that regulates ROS generation. The purpose of this study was to investigate whether NQO1 modulates the renal I/R injury (IRI) associated with NADPH oxidase (NOX)-derived ROS production in an animal model. We analyzed renal function, oxidative stress, and tubular apoptosis after IRI. NQO1-/- mice showed increased blood urea nitrogen and creatinine levels, tubular damage, oxidative stress, and apoptosis. In the kidneys of NQO1-/- mice, the cellular NADPH/NADP+ ratio was significantly higher and NOX activity was markedly higher than in those of NQO1+/+ mice. The activation of NQO1 by β-lapachone (βL) significantly improved renal dysfunction and reduced tubular cell damage, oxidative stress, and apoptosis by renal I/R. Moreover, the βL treatment significantly lowered the cellular NADPH/NADP+ ratio and dramatically reduced NOX activity in the kidneys after IRI. From these results, it was concluded that NQO1 has a protective role against renal injury induced by I/R and that this effect appears to be mediated by decreased NOX activity via cellular NADPH/NADP+ modulation. These results provide convincing evidence that NQO1 activation might be beneficial for ameliorating renal injury induced by I/R.
Keyword
β-LapachoneFree radicalsIschemia/reperfusion injuryNADPH oxidaseNQO1
ISSN
0891-5849
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.freeradbiomed.2013.10.817
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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