Lacibacter daechungensis sp. nov., isolated from deep freshwater of a reservoir

Abstract

A Gram-stain-negative, aerobic, motile by gliding, non-spore-forming, rod-shaped, orange-pigmented bacterium, designated strain H32-4T, was isolated from 32 m deep water of Daechung reservoir in Daejeon, Republic of Korea. Based on the nucleotide sequence of the 16S rRNA gene, the closest neighbouring type strain was Lacibacter cauensis NJ-8T with which strain H32-4T shared 98.9 % sequence similarity. The most abundant fatty acids in whole cells of strain H32-4T were C15: 0 iso (40.6 %), C17: 0 iso 3-OH (22.4 %), summed feature 3 (C16: 1ω7c and/or C16: 1ω6c; 9.3 %) and C15: 0 (6.6 %). The predominant menaquinone was MK-7. The G+C content of the genomic DNA of strain H32-4T was 45.7 mol%. Thus, these combined genotypic and phenotypic data supported the conclusion that strain H32-4T represents a novel species of the genus Lacibacter, for which the name Lacibacter daechungensis sp. nov. is proposed. The type strain is H32-4T (= KCTC 32395T = JCM 19172T).