Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation

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dc.contributor.authorJ Zhou-
dc.contributor.authorC Wang-
dc.contributor.authorS H Yoon-
dc.contributor.authorH J Jang-
dc.contributor.authorEui Sung Choi-
dc.contributor.authorS W Kim-
dc.date.accessioned2017-04-19T09:48:04Z-
dc.date.available2017-04-19T09:48:04Z-
dc.date.issued2014-
dc.identifier.issn0168-1656-
dc.identifier.uri10.1016/j.jbiotec.2013.11.009ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11735-
dc.description.abstractGeraniol, a monoterpene alcohol, has versatile applications in the fragrance industry, pharmacy and agrochemistry. Moreover, geraniol could be an ideal gasoline alternative. In this study, recombinant overexpression of geranyl diphosphate synthase and the bottom portion of a foreign mevalonate pathway in Escherichia coli MG1655 produced 13.3. mg/L of geraniol. Introduction of Ocimum basilicum geraniol synthase increased geraniol production to 105.2. mg/L. However, geraniol production encountered a loss from its endogenous dehydrogenization and isomerization into other geranoids (nerol, neral and geranial). Three E. coli enzymes (YjgB, YahK and YddN) were identified with high sequence identity to plant geraniol dehydrogenases. YjgB was demonstrated to be the major one responsible for geraniol dehydrogenization. Deletion of yjgB increased geraniol production to 129.7. mg/L. Introduction of the whole mevalonate pathway for enhanced building block synthesis from endogenously synthesized mevalonate improved geraniol production up to 182.5. mg/L in the yjgB mutant after 48. h of culture, which was a double of that obtained in the wild type control (96.5. mg/L). Our strategy for improving geraniol production in engineered E. coli should be generalizable for addressing similar problems during metabolic engineering.-
dc.publisherElsevier-
dc.titleEngineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation-
dc.title.alternativeEngineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation-
dc.typeArticle-
dc.citation.titleJournal of Biotechnology-
dc.citation.number1-
dc.citation.endPage50-
dc.citation.startPage42-
dc.citation.volume169-
dc.contributor.affiliatedAuthorEui Sung Choi-
dc.contributor.alternativeNameZhou-
dc.contributor.alternativeNameWang-
dc.contributor.alternativeName윤상활-
dc.contributor.alternativeName장희정-
dc.contributor.alternativeName최의성-
dc.contributor.alternativeName김선원-
dc.identifier.bibliographicCitationJournal of Biotechnology, vol. 169, no. 1, pp. 42-50-
dc.identifier.doi10.1016/j.jbiotec.2013.11.009-
dc.subject.keywordE. coli-
dc.subject.keywordGeraniol-
dc.subject.keywordGeraniol dehydrogenation-
dc.subject.keywordMevalonate pathway-
dc.subject.keywordMonoterpene-
dc.subject.localEscherichia coli.-
dc.subject.localescherichia coli-
dc.subject.localEscherichia Coli-
dc.subject.localEscherichia coli-
dc.subject.localE.coli-
dc.subject.localescherichia coil-
dc.subject.localE. coli-
dc.subject.localE. Coli-
dc.subject.localGeraniol-
dc.subject.localGeraniol dehydrogenation-
dc.subject.localMevalonate pathway-
dc.subject.localmevalonate pathway-
dc.subject.localMevalonate Pathway-
dc.subject.localMonoterpene-
dc.description.journalClassY-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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