EC-18, a synthetic monoacetyldiglyceride (1-palmitoyl-2-linoleoyl-3- acetylglycerol), attenuates the asthmatic response in an aluminum hydroxide/ovalbumin-induced model of asthma

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Title
EC-18, a synthetic monoacetyldiglyceride (1-palmitoyl-2-linoleoyl-3- acetylglycerol), attenuates the asthmatic response in an aluminum hydroxide/ovalbumin-induced model of asthma
Author(s)
In Sik Shin; Na Rae Shin; Chan Mi Jeon; Ok-Kyoung Kwon; K Y Sohn; T S Lee; Jae Wha KimKyung Seop AhnSei-Ryang Oh
Bibliographic Citation
International Immunopharmacology, vol. 18, no. 1, pp. 116-123
Publication Year
2014
Abstract
EC-18 is a synthetic monoacetyldiaglyceride that is a major constituent in antlers of Sika deer (Cervus nippon Temmenick). In this study, we evaluated the protective effects of EC-18 on Th2-type cytokines, eosinophil infiltration, and other factors in an aluminum hydroxide/ovalbumin (OVA)-induced murine asthma model. Mice were sensitized on days 0 and 14 by intraperitoneal injection of OVA with aluminum hydroxide. On days 21, 22 and 23 after the initial sensitization, the mice received an airway challenge with OVA for 1 h using an ultrasonic nebulizer. EC-18 was administered to mice by oral gavage at doses of 30 mg/kg and 60 mg/kg once daily from day 18 to 23. Methacholine responsiveness was measured 24 h after the final OVA challenge, and the bronchoalveolar lavage fluid (BALF) was collected 48 h after the final OVA challenge. EC-18 significantly reduced methacholine responsiveness, T helper type 2 (Th2) cytokines, eotaxin-1, immunoglobulin (Ig) E, IgG, and the number of inflammatory cells. In addition, EC-18-treated mice exhibited the reduction in the expression of inducible nitric oxide synthase (iNOS) in lung tissue. In the histological analysis using hematoxylin-eosin stain and periodic acid-Schiff stain, EC-18 attenuated the infiltration of inflammatory cells into the airway and reduced the level of mucus production. Our results showed that EC-18 effectively suppressed the asthmatic response induced by OVA challenge. These effects were considered to be associated with iNOS suppression. In conclusion, this study suggests that EC-18 may be a therapeutic agent for allergic asthma
Keyword
AsthmaCytokinesEC-18Inducible nitric oxide synthaseMonoacetyldiacylglyceride
ISSN
1567-5769
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.intimp.2013.11.006
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
Ochang Branch Institute > 1. Journal Articles
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