Digital mRNA profiling of N-glycosylation gene expression in recombinant Chinese hamster ovary cells treated with sodium butyrate

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dc.contributor.authorS M Lee-
dc.contributor.authorYeon Gu Kim-
dc.contributor.authorEun Gyo Lee-
dc.contributor.authorG M Lee-
dc.date.accessioned2017-04-19T09:49:30Z-
dc.date.available2017-04-19T09:49:30Z-
dc.date.issued2014-
dc.identifier.issn0168-1656-
dc.identifier.uri10.1016/j.jbiotec.2013.12.001ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11754-
dc.description.abstractTo understand the effects of sodium butyrate (NaBu) on protein glycosylation, recombinant Chinese hamster ovary (rCHO) cells producing Fc-fusion glycoprotein were subjected to 3. mM NaBu. The addition of NaBu to the cultures reduced the relative proportion of acidic isoforms and sialic acid content of the glycoprotein. Fifty-two N-glycosylation-related gene expressions were also assessed by the NanoString nCounter system, which can provide a direct digital readout using custom-designed color-coded probes. Among them, ten genes (. ugp, slc35a2, ganc, man1a, man1c, mgat5a, st3gal5, glb1, neu1, and neu3) were up-regulated and three genes (. b4galt2, st3gal3, and neu2) were down-regulated significantly. Altered expression patterns in st3gal3, neu1, and neu3, which have roles in the sialic acid biosynthesis pathway, correlated with reduced sialic acid content of the glycoprotein by NaBu. Taken together, the results obtained in this study provide a better understanding of the detrimental effect of NaBu on N-glycosylation in rCHO cells.-
dc.publisherElsevier-
dc.titleDigital mRNA profiling of N-glycosylation gene expression in recombinant Chinese hamster ovary cells treated with sodium butyrate-
dc.title.alternativeDigital mRNA profiling of N-glycosylation gene expression in recombinant Chinese hamster ovary cells treated with sodium butyrate-
dc.typeArticle-
dc.citation.titleJournal of Biotechnology-
dc.citation.number1-
dc.citation.endPage60-
dc.citation.startPage56-
dc.citation.volume171-
dc.contributor.affiliatedAuthorYeon Gu Kim-
dc.contributor.affiliatedAuthorEun Gyo Lee-
dc.contributor.alternativeName이상민-
dc.contributor.alternativeName김연구-
dc.contributor.alternativeName이은교-
dc.contributor.alternativeName이균민-
dc.identifier.bibliographicCitationJournal of Biotechnology, vol. 171, no. 1, pp. 56-60-
dc.identifier.doi10.1016/j.jbiotec.2013.12.001-
dc.subject.keywordCHO cells-
dc.subject.keywordGene expression-
dc.subject.keywordN-Glycosylation-
dc.subject.keywordNanoString nCounter system-
dc.subject.keywordSodium butyrate-
dc.subject.localCHO cell-
dc.subject.localCHO cells-
dc.subject.localGene Expression-
dc.subject.localGene expression-
dc.subject.localgene expression-
dc.subject.localn-glycosylation-
dc.subject.localN-Glycosylation-
dc.subject.localN-glycosylation-
dc.subject.localNanostring nCounter system-
dc.subject.localNanostring nCounter syste-
dc.subject.localnanostring nCounter system-
dc.subject.localNanoString nCounter system-
dc.subject.localSodium butyrate-
dc.subject.localsodium butyrate-
dc.subject.localsodium butyrate (NaB)-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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