Suppression of PTRF alleviates the polymicrobial sepsis induced by cecal ligation and puncture in mice

Cited 9 time in scopus
Metadata Downloads
Title
Suppression of PTRF alleviates the polymicrobial sepsis induced by cecal ligation and puncture in mice
Author(s)
Y Zheng; Seon-Jin Lee; X Liang; S Wei; H G Moon; Y Jin
Bibliographic Citation
Journal of Infectious Diseases, vol. 208, no. 11, pp. 1803-1812
Publication Year
2013
Abstract
Background. Sepsis and sepsis-associated organ failure are devastating conditions. Understanding the detailed cellular/molecular mechanisms involved in sepsis should lead to the identification of novel therapeutic targets. Methods. Cecal ligation and puncture (CLP) was used as a polymicrobial sepsis model in vivo to determine mortality and end-organ damage. Macrophages were adopted as the cellular model in vitro for mechanistic studies. Results. PTRF+/- mice survived longer and suffered less organ damage after CLP. Reductions in nitric oxide (NO) and iNOS biosynthesis were observed in plasma, macrophages, and vital organs in the PTRF+/- mice. Using an acute sepsis model after CLP, we found that iNOS-/- mice had a comparable level of survival as the PTRF+/- mice. Similarly, polymerase I transcript release factor (PTRF) deficiency resulted in decreased iNOS and NO/ROS production in macrophages in vitro. Mechanistically, lipopolysaccharide (LPS) enhanced the co-localization and interaction between PTRF and TLR4 in lipid rafts. Deletion of PTRF blocked formation of the TLR4/Myd88 complex after LPS. Consistent with this, lack of PTRF impaired the TLR4 signaling, as shown by the decreased p-JNK, p-ERK, and p-p38, which are upstream factors involved in iNOS transcription. Conclusion. PTRF is a crucial regulator of TLR4 signaling in the development of sepsis.
Keyword
MacrophageNitric oxidePTRFROSSepsisCLPTLR4
ISSN
0022-1899
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/infdis/jit364
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.