Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain

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Title
Production of isobutanol from crude glycerol by a genetically-engineered Klebsiella pneumoniae strain
Author(s)
Baek Rock OhSun-Yeon Heo; S M Lee; Won-Kyung Hong; Jang Min Park; You Ree Jung; D H Kim; Jung Hoon SohnJeong-Woo SeoChul Ho Kim
Bibliographic Citation
Biotechnology Letters, vol. 36, no. 1, pp. 57-62
Publication Year
2014
Abstract
Klebsiella pneumoniae was engineered to produce 2-butanol from crude glycerol as a sole carbon source by expressing acetolactate synthase (ilvIH), keto-acid reducto-isomerase (ilvC) and dihydroxy-acid dehydratase (ilvD) from K. pneumoniae, and α-ketoisovalerate decarboxylase (kivd) and alcohol dehydrogenase (adhA) from Lactococcus lactis. Engineered K. pneumonia, {increment}ldhA/pBR-iBO (ilvIH-ilvC-ilvD-kivd-adhA), produced 2-butanol (160 mg l-1) from crude glycerol. To increase the yield of 2-butanol, we eliminated the 2,3-butanediol pathway from the recombinant strain by inactivating α-acetolactate decarboxylase (adc). This further engineering step improved the yield of 2-butanol from 160 to 320 mg l-1. This represents the first successful attempt to produce 2-butanol from crude glycerol.
Keyword
2-Butanol/isobutanolCrude glycerolGenetic engineeringKlebsiella pneumoniae
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-013-1333-0
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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