Fourier transform infrared (FT-IR) spectroscopy of genomic DNA to discriminate F1 progenies from their paternal lineage of Chinese cabbage (Brassica rapa subsp. pekinensis)

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dc.contributor.authorS Y Song-
dc.contributor.authorEun-Yee Jie-
dc.contributor.authorMyung-Suk Ahn-
dc.contributor.authorI H Lee-
dc.contributor.authorI S Nou-
dc.contributor.authorB W Min-
dc.contributor.authorSuk Weon Kim-
dc.date.accessioned2017-04-19T09:51:13Z-
dc.date.available2017-04-19T09:51:13Z-
dc.date.issued2014-
dc.identifier.issn1380-3743-
dc.identifier.uri10.1007/s11032-013-9963-4ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11848-
dc.description.abstractFourier transform infrared spectroscopy (FT-IR) spectroscopy in combination with multivariate analysis was used to discriminate two different F1 hybrid lines from their parental inbred lines. Genomic DNA was isolated from leaves of three parental lines and two F1 hybrid lines of Brassica campestris subsp. pekinensis. Purified genomic DNA was analyzed by FT-IR spectroscopy in the spectral region from 4,000 to 400 cm-1. FT-IR spectra confirmed typical spectral differences between the frequency regions of N-H stretching (amide I) and C=O stretching vibrations (amide II) as well as PO2 - ionized asymmetric and symmetric stretching. Principal component analysis was able to discriminate between F1 hybrid progenies depending on their parental lineages, even though they share the same maternal background. Partial least squares discriminant analysis gave a more clear discrimination between the two parental lines and their hybrid progenies. These FT-IR spectral differences might be directly related to subtle changes in the base functional group and backbone structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for FT-IR spectral-based rapid diagnosis, selection, and discrimination of parental lines from their progenies. Furthermore, this technique could be applied to test purity in the hybrid seed industry.-
dc.publisherSpringer-
dc.titleFourier transform infrared (FT-IR) spectroscopy of genomic DNA to discriminate F1 progenies from their paternal lineage of Chinese cabbage (Brassica rapa subsp. pekinensis)-
dc.title.alternativeFourier transform infrared (FT-IR) spectroscopy of genomic DNA to discriminate F1 progenies from their paternal lineage of Chinese cabbage (Brassica rapa subsp. pekinensis)-
dc.typeArticle-
dc.citation.titleMolecular Breeding-
dc.citation.number2-
dc.citation.endPage464-
dc.citation.startPage453-
dc.citation.volume33-
dc.contributor.affiliatedAuthorEun-Yee Jie-
dc.contributor.affiliatedAuthorMyung-Suk Ahn-
dc.contributor.affiliatedAuthorSuk Weon Kim-
dc.contributor.alternativeName송승엽-
dc.contributor.alternativeName지은이-
dc.contributor.alternativeName안명숙-
dc.contributor.alternativeName이인호-
dc.contributor.alternativeName노일섭-
dc.contributor.alternativeName민병완-
dc.contributor.alternativeName김석원-
dc.identifier.bibliographicCitationMolecular Breeding, vol. 33, no. 2, pp. 453-464-
dc.identifier.doi10.1007/s11032-013-9963-4-
dc.subject.keywordChinese cabbage-
dc.subject.keywordDiscrimination-
dc.subject.keywordFT-IR-
dc.subject.keywordGenomic DNA-
dc.subject.keywordMultivariate analysis-
dc.subject.keywordProgeny-
dc.subject.localChinese cabbage (Brassica rapa L.)-
dc.subject.localChinese cabbage-
dc.subject.localchinese cabbage-
dc.subject.localDiscrimination-
dc.subject.localfourier transform infared spectroscopy-
dc.subject.localFourier transform-infrared spectroscopy-
dc.subject.localFourier transformation infrared (FT-IR) spectroscopy-
dc.subject.localFT-IR spectroscopy-
dc.subject.localFourier transform infrared spectroscopy (FT-IR)-
dc.subject.localFT-IR-
dc.subject.localFourier transformation - Infrared spectroscopy-
dc.subject.localFourier transformation - infrared spectroscopy-
dc.subject.localFTIR-
dc.subject.localfourier transformation infrared spectroscopy-
dc.subject.localFourier transform infrared spectroscopy-
dc.subject.localFourier transformation infrared spectroscopy (FT-IR)-
dc.subject.localFT-IR (Fourier transform infrared spectroscopy)-
dc.subject.localFourier transform IR-
dc.subject.localFourier transform-infrared spectroscopy (FT-IR)-
dc.subject.localgenomic DNA-
dc.subject.localGenomic DNA-
dc.subject.localMultivariate analysis-
dc.subject.localmultivariate analysis-
dc.subject.localProgeny-
dc.subject.localprogenies-
dc.subject.localprogeny-
dc.description.journalClassY-
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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