Homogeneous fluorescence resonance energy transfer immunoassay for the determination of zearalenone

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Title
Homogeneous fluorescence resonance energy transfer immunoassay for the determination of zearalenone
Author(s)
T Li; Bo Bae Kim; W B Shim; Ju Young Byun; D H Chung; Yong Beom Shin; M G Kim
Bibliographic Citation
Analytical Letters, vol. 47, no. 3, pp. 453-464
Publication Year
2014
Abstract
This study demonstrates the use of antigen-antibody binding for the detection of zearalenone. Based on the principle of the fluorescence resonance energy transfer (FRET) phenomenon between antibody and antigen, an immunoassay, in which zearalenone coupled with the anti-zearalenone antibody, was developed, optimized, and applied. Owing to intrinsic fluorescence properties in basic pH conditions with the optimal cationic surfactant, anti-zearalenone and zearalenone played roles as the respective donor and acceptor in the FRET immunoassay. As the concentration of analyte increased, the antigen/antibody emission intensity ratio (I430nm/I350nm) was enhanced due to larger amounts of zearalenone/anti-zearalenone complexes. This assay, based on the ratio of intensities (I430nm/I350 nm), displayed high specificity and sensitivity with a detection limit of 0.8 ng mL-1 for zearalenone. The results obtained from analysis of spiked wheat grain samples were found to be in good agreement with those obtained by employing a direct competitive enzyme-linked immunosorbent assay. The label-free, noncompetitive, and homogeneous FRET immunoassay strategy served as a powerful tool for the simple, rapid, and sensitive quantitative determination of zearalenone in food and feed matrices.
Keyword
AntibodyFRETImmunoassaypHSurfactantZearalenone
ISSN
0003-2719
Publisher
T&F (Taylor & Francis)
DOI
http://dx.doi.org/10.1080/00032719.2013.843186
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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