Hymenobacter ruber sp. nov., isolated from grass soil

Abstract

A taxonomic study using a polyphasic approach was performed on a Gram-stain-negative, aerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain PB156T, isolated from grass soil. Comparative 16S rRNA gene sequence studies showed that the isolate was clearly affiliated with the phylum Bacteroidetes, and most closely related toHymenobacter soli PB17T, Hymenobacter antarcticus VUG-A42aaT andHymenobacter glaciei VUG-A130T, showing 96.4, 96.2 and 95.9 % 16S rRNA gene sequence similarity, respectively, while all other species of the genus shared only 89.3-95.2 % similarity. The main polyamine present was sym-homospermidine. The predominant menaquinone was MK-7. The major fatty acids were C15: 0 iso, summed feature 3 (C16: 1ω6c and/or C16: 1ω7c/t), C16: 1ω5c and C15: 0 anteiso. The G+C content of the genomic DNA of strain PB156T was 61.7 mol%. The combined genotypic and phenotypic data supported the conclusion that strain PB156T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter ruber sp. nov. is proposed. The type strain is PB156T (= KCTC 32477T = JCM 19433T).