Inhibition effect of flavonoid compounds against neuraminidase expressed in Pichia pastoris = Flavonoid 화합물들의 뉴라미니데이즈 저해효과

Cited 5 time in scopus
Metadata Downloads
Title
Inhibition effect of flavonoid compounds against neuraminidase expressed in Pichia pastoris = Flavonoid 화합물들의 뉴라미니데이즈 저해효과
Author(s)
T T H Nguyen; H K Kang; Young-Min Kim; D Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 19, no. 1, pp. 70-75
Publication Year
2014
Abstract
Neuraminidase (NA) is one of the two glycoproteins on the surface of influenza virus, which cleaves terminal sialic acid residues and facilitates the release of virions from infected cells. The recombinant NA from H5N1 influenza virus strain A/Vietnam/1203/04 was expressed in Pichia pastoris X33 as a 45 kDa protein that displayed a K m of 9.96 ± 1.26 μM with fluorogenic substrate, 2′-(4-methylumbelliferyl)-α-D-N-acetyl neuraminic acid. Partially purified NA was used for the inhibition and kinetic assays with eight flavonoid compounds and gallic acid. Among them, gallocatechin gallate (GCG) showed the best inhibition against NA with the IC50 of 8.98 ± 0.46 μM and showed a competitive inhibition pattern with K i value of 8.34 ± 0.25 μM. In molecular docking experiments, GCG displayed a binding energy of -13.71 kcal/mol to the active site of NA and the galloyl moiety was required for NA inhibition activity.
Keyword
catechinflavonoidH5N1molecular dockingneuraminidasePichia pastoris
ISSN
1226-8372
Publisher
South Korea
DOI
http://dx.doi.org/10.1007/s12257-013-0599-3
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.