Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells

Cited 34 time in scopus
Metadata Downloads
Title
Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells
Author(s)
Sun-Uk KimYoung-Ho Park; J M Kim; H N Sun; I S Song; S M Huang; S H Lee; J I Chae; S Hong; S S Choi; S C Choi; T H Lee; S W Kang; S G Rhee; Kyu Tae Chang; S H Lee; Dae Yeul Yu; D S Lee
Bibliographic Citation
Stem Cells, vol. 32, no. 4, pp. 998-1011
Publication Year
2014
Abstract
Redox balance has been suggested as an important determinant of "stemness" in embryonic stem cells (ESCs). In this study, we demonstrate that peroxiredoxin (Prx) plays a pivotal role in maintenance of ESC stemness during neurogenesis through suppression of reactive oxygen species (ROS)-sensitive signaling. During neurogenesis, Prx I and Oct4 are expressed in a mutually dependent manner and their expression is abruptly downregulated by an excess of ROS. Thus, in Prx I-/- or Prx II-/- ESCs, rapid loss of stemness can occur due to spontaneous ROS overload, leading to their active commitment into neurons; however, stemness is restored by the addition of an antioxidant or an inhibitor of c-Jun N-terminal kinase (JNK). In addition, Prx I and Prx II appear to have a tight association with the mechanism underlying the protection of ESC stemness in developing teratomas. These results suggest that Prx functions as a protector of ESC stemness by opposing ROS/JNK cascades during neurogenesis. Therefore, our findings have important implications for understanding of maintenance of ESC stemness through involvement of antioxidant enzymes and may lead to development of an alternative stem cell-based therapeutic strategy for production of high-quality neurons in large quantity.
Keyword
Embryonic stem cellsNeurogenesisPeroxiredoxinReactive oxygen speciesAntioxidants
ISSN
1066-5099
Publisher
Wiley
DOI
http://dx.doi.org/10.1002/stem.1593
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.