Development of transgenic cucumbers expressing Arabidopsis Nit gene = 애기장대 Nit유전자 발현 오이 형질전화체 개발

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dc.contributor.authorHyun A Jang-
dc.contributor.authorK M Lim-
dc.contributor.authorHyun A Kim-
dc.contributor.authorY I Park-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorP S Choi-
dc.date.accessioned2017-04-19T09:52:13Z-
dc.date.available2017-04-19T09:52:13Z-
dc.date.issued2013-
dc.identifier.issn1229-2818-
dc.identifier.uri10.5010/JPB.2013.40.4.198ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11911-
dc.description.abstractTo produce transgenic cucumber expressing Nit gene coffering abiotic resistance, the cotyledonary-node explants of cucumber (cv. Eunsung) were inoculated with A. tumefaciens transformed with pPZP211 or pCAMBIA2300 carrying Nit gene, that has cis-acting element involved in resistance to various abiotic environmental stresses. After co-cultivation, the procedures of selection, shoot initiation, shoot elongation, and plant regeneration were followed by cotyledonary-node transformation method (CTM, Jang et al. 2011). The putative transgenic plants were selected when shoots were grown to a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 100 mg/L paromomycin as a selectable agent. The confirmation of transgenic cucumber was based on the genomic PCR, Southern blot analysis, RT-PCR, and Northern blot analysis. A 105 shoots (4.12%) selected from the selection mediums were obtained from 2,547 explants inoculated. Of them, putative transgenic plants were only confirmed with 45 plants (1.77%) by genomic PCR analysis. Transgenic plants showed that the Nit genes integrated into each genome of 39 plants (1.53%) by Southern blot analysis, and the expression of gene integrated into cucumber genome was only confirmed at 6 plants (0.24%) by RT-PCR and Northern blot analysis. These results lead us to speculate that the genes were successfully integrated and expressed in each genome of transgenic cucumber.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDevelopment of transgenic cucumbers expressing Arabidopsis Nit gene = 애기장대 Nit유전자 발현 오이 형질전화체 개발-
dc.title.alternativeDevelopment of transgenic cucumbers expressing Arabidopsis Nit gene-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number4-
dc.citation.endPage202-
dc.citation.startPage198-
dc.citation.volume40-
dc.contributor.affiliatedAuthorHyun A Jang-
dc.contributor.affiliatedAuthorHyun A Kim-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.alternativeName장현아-
dc.contributor.alternativeName임가민-
dc.contributor.alternativeName김현아-
dc.contributor.alternativeName박연일-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName최필선-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 40, no. 4, pp. 198-202-
dc.identifier.doi10.5010/JPB.2013.40.4.198-
dc.subject.keywordAbiotic gene-
dc.subject.keywordAgrobacterium-
dc.subject.keywordNit gene-
dc.subject.keywordTransgenic cucumber-
dc.subject.localAbiotic gene-
dc.subject.localAgrobacterium-
dc.subject.localagrobacterium-
dc.subject.localAgrobact erium-
dc.subject.localNit gene-
dc.subject.localTransgenic cucumber-
dc.subject.localtransgenic cucumber-
dc.description.journalClassN-
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