Inhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference

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dc.contributor.authorH C Chung-
dc.contributor.authorV G Nguyen-
dc.contributor.authorH J Moon-
dc.contributor.authorHye Kwon Kim-
dc.contributor.authorSeong-Jun Park-
dc.contributor.authorJ H Lee-
dc.contributor.authorM G Choi-
dc.contributor.authorA R Kim-
dc.contributor.authorB K Park-
dc.date.accessioned2017-04-19T09:52:14Z-
dc.date.available2017-04-19T09:52:14Z-
dc.date.issued2014-
dc.identifier.issn0934-0874-
dc.identifier.uri10.1111/tri.12219ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11912-
dc.description.abstractTo effectively suppress porcine endogenous retroviruses (PERV)s, RNAi technique was utilized. RNAi is the up-to-date skill for gene knockdown which simultaneously multitargets both gag and pol genes critical for replication of PERVs. Previously, two of the most effective siRNAs (gag2, pol2) were found to reduce the expression of PERVs. Concurrent treatment of these two siRNAs (gag2+pol2) showed knockdown efficiency of up to 88% compared to negative control. However, despite the high initial knockdown efficiency 48 h after transfection caused by siRNA, it may only be a transient effect of suppressing PERVs. The multitargeting vector was designed, containing both gag and pol genes and making use of POL II miR Expression Vector, which allowed for persistent and multiple targeting. This is the latest shRNA system technique expressing and targeting like miRNA. Through antibiotics resistance characteristics utilizing this vector, miRNA-transfected PK15 cells (gag2-pol2) were selected during 10 days. An 88.1% reduction in the level of mRNA expression was found. In addition, we performed RT-activity analysis and fluorescence in situ hybridization assay, and it demonstrated the highest knockdown efficiency in multitargeting (gag2+pol2) miRNA group. Therefore, according to the results above, gene knockdown system (siRNA and shRNA) through multitargeting strategy could effectively inhibit PERVs.-
dc.publisherWiley-
dc.titleInhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference-
dc.title.alternativeInhibition of porcine endogenous retrovirus in PK15 cell line by efficient multitargeting RNA interference-
dc.typeArticle-
dc.citation.titleTransplant International-
dc.citation.number1-
dc.citation.endPage105-
dc.citation.startPage96-
dc.citation.volume27-
dc.contributor.affiliatedAuthorHye Kwon Kim-
dc.contributor.affiliatedAuthorSeong-Jun Park-
dc.contributor.alternativeName정희천-
dc.contributor.alternativeNameNguyen-
dc.contributor.alternativeName문형준-
dc.contributor.alternativeName김혜권-
dc.contributor.alternativeName박성준-
dc.contributor.alternativeName이지훈-
dc.contributor.alternativeName최민경-
dc.contributor.alternativeName김아름-
dc.contributor.alternativeName박봉균-
dc.identifier.bibliographicCitationTransplant International, vol. 27, no. 1, pp. 96-105-
dc.identifier.doi10.1111/tri.12219-
dc.subject.keywordinhibition-
dc.subject.keywordmultitargeting-
dc.subject.keywordPK15 cells-
dc.subject.keywordporcine endogenous retrovirus-
dc.subject.keywordRNA interference-
dc.subject.localinhibition-
dc.subject.localInhibition-
dc.subject.localMulti-targeting-
dc.subject.localmultitargeting-
dc.subject.localPK15 cells-
dc.subject.localporcine endogenous retrovirus-
dc.subject.localporcine endogenous retroviruses-
dc.subject.localRNA interference-
dc.subject.localRNA Interference-
dc.description.journalClassY-
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