Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin

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Title
Highly stable colorimetric aptamer sensors for detection of ochratoxin A through optimizing the sequence with the covalent conjugation of hemin
Author(s)
Jayeon Lee; Chang Hoon Jeon; S J Ahn; Tai Hwan Ha
Bibliographic Citation
Analyst, vol. 139, no. 7, pp. 1622-1627
Publication Year
2014
Abstract
Optimization of hairpin DNA is introduced to detect ochratoxin A (OTA) by chemically conjugating its cofactor, hemin, toward the 5′-end. The newly designed OTA aptasensor showed enhanced stability and sensitivity, thereby lowering the detection limit to an ∼1 nM level. Furthermore, an optimal spacer for hemin conjugation was investigated for stable responses toward very diluted OTA solutions.
ISSN
0003-2654
Publisher
Royal Soc Chem
DOI
http://dx.doi.org/10.1039/c3an01639k
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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