Characterisation of Pseudomonas aeruginosa related to bovine mastitis

Cited 18 time in scopus
Metadata Downloads
Title
Characterisation of Pseudomonas aeruginosa related to bovine mastitis
Author(s)
H R Park; Min Ki Hong; S Y Hwang; Y K Park; K H Kwon; J W Yoon; S Shin; J H Kim; Y H Park
Bibliographic Citation
Acta Veterinaria Hungarica, vol. 62, no. 1, pp. 1-12
Publication Year
2014
Abstract
Pseudomonas aeruginosa is one of the causative pathogens of bovine mastitis. Most P. aeruginosa strains possess the type III secretion system (TTSS), which may increase somatic cell counts (SCCs) in milk from mastitis-affected cows. Moreover, most of P. aeruginosa cells can form biofilms, thereby reducing antibiotic efficacy. In this study, the presence and effect of TTSS-related genotypes on increase of SCCs among 122 P. aeruginosa isolates obtained from raw milk samples from mastitis-affected cows and their antibiotic susceptibility at planktonic and biofilm status were investigated. Based on the presence of TTSS-related genes a total of 82.7% of the isolates were found to harbour exoU and/or exoS genes, including the invasive (exoU-/exoS+, 69.4%), cytotoxic (exoU+/exoS-, 8.3%) and cytotoxic/invasive strains (exoU+/exoS+, 5.0%). Milk containing exoS-positive isolates had higher SCCs than those containing exoS-negative isolates. The majority of isolates showed gentamicin, amikacin, meropenem and ciprofloxacin susceptibility at planktonic status. However, the susceptibility was decreased at the biofilm status. Based on minimum biofilm eradication concentration (MBEC)/minimum inhibitory concentration (MIC) ratios, the range of change in antibiotic susceptibility varied widely depending on the antibiotics (from ≥ 3.1-fold to ≥ 475.0-fold). In conclusion, most P. aeruginosa isolates studied here had a genotype related to increase in SCCs. The efficiency of antibiotic therapy against P. aeruginosa-related bovine mastitis could be improved by analysing both the MBEC and the MIC of isolates.
Keyword
minimum biofilm eradication concentration (MBEC)Pseudomonas aeruginosatype three secretion system (TTSS)biofilmbovine mastitis
ISSN
0236-6290
Publisher
Akademiai Kiado Zrt
DOI
http://dx.doi.org/10.1556/AVet.2013.054
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.