The EF-hand calcium-binding protein tescalcin is a potential oncotarget in colorectal cancer

Cited 23 time in scopus
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Title
The EF-hand calcium-binding protein tescalcin is a potential oncotarget in colorectal cancer
Author(s)
Yun Hee Kang; S R Han; Jong-Tae KimSeon-Jin LeeYoung Il Yeom; Jeong Ki Min; Chul Ho LeeJae Wha KimSuk Ran Yoon; D Y Yoon; K S Hong; G S Hwang; H C Kim; Y H Lee; Hee Gu Lee
Bibliographic Citation
Oncotarget, vol. 5, no. 8, pp. 2149-2160
Publication Year
2014
Abstract
Tescalcin (TESC) is an EF-hand calcium binding protein that is differentially expressed in several tissues, however it is not reported that the expression and functional roles of TESC in colorectal cancer. Levels of messenger RNA (mRNA) and protein expression of TESC in colorectal cancer tissues were assessed using RT-PCR, real time PCR, immunohistochemistry, and clinicopathologic analyses. Quantitative analysis of TESC levels in serum specimens was performed using sandwich ELISA. Colorectal cancer cells transfected with TESC small interfering RNA and short hairpin RNA were examined in cell proliferation assays, phospho-MAPK array, and mouse xenograft models. Here we demonstrated that TESC is overexpressed in colorectal cancer (CRC), but was not expressed in normal mucosa and premalignant dysplastic lesions. Furthermore, serum TESC levels were elevated in patients with CRC. Knockdown of TESC inhibited the Akt-dependent NF-κB pathway and decreased cell survival in vitro. Depletion of TESC reduced tumor growth in a CRC xenograft model. Thus, TESC is a potential diagnostic marker and oncotarget in colorectal cancer.
Keyword
Cell growthColorectal cancerNF-κBTescalcinTumor growth
ISSN
1949-2553
Publisher
Impact Journals
DOI
http://dx.doi.org/10.18632/oncotarget.1851
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Personalized Genomic Medicine Research Center > 1. Journal Articles
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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