Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro

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Title
Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro
Author(s)
S W Park; J Kim; Jong Lyul Park; J Y Ko; I Im; H S Do; H Kim; N T Tran; S H Lee; Yong Sung Kim; Yee Sook Cho; D R Lee; Y M Han
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 446, no. 2, pp. 493-498
Publication Year
2014
Abstract
Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vitro differentiation into specialized cell types. Out of 9 imprinted genes with single nucleotide polymorphisms, mono-allelic expression for three imprinted genes (H19, KCNQ1OT1, and IPW), and bi- or partial-allelic expression for three imprinted genes (OSBPL5, PPP1R9A, and RTL1) were stably retained in H9-hESCs throughout differentiation, representing imprinting stability. Three imprinted genes (KCNK9, ATP10A, and SLC22A3) showed a loss and a gain of imprinting in a lineage-specific manner during differentiation. Changes in allelic expression of imprinted genes were observed in another hESC line during in vitro differentiation. These findings indicate that the allelic expression of imprinted genes may be vulnerable in a lineage-specific manner in human pluripotent stem cells during differentiation.
Keyword
Allele-specific expressionHuman pluripotent stem cellsImprinted genesIn vitro differentiation
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2014.02.141
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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