Development of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use

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dc.contributor.authorM Nam-
dc.contributor.authorJ S Kim-
dc.contributor.authorSeungmo Lim-
dc.contributor.authorC Y Park-
dc.contributor.authorJ G Kim-
dc.contributor.authorH S Choi-
dc.contributor.authorH S Lim-
dc.contributor.authorJae Sun Moon-
dc.contributor.authorS H Lee-
dc.date.accessioned2017-04-19T09:52:58Z-
dc.date.available2017-04-19T09:52:58Z-
dc.date.issued2014-
dc.identifier.issnI000-0113-
dc.identifier.uri10.5423/PPJ.OA.08.2013.0084ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11975-
dc.description.abstractA large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleDevelopment of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use-
dc.title.alternativeDevelopment of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use-
dc.typeArticle-
dc.citation.titlePlant Pathology Journal-
dc.citation.number1-
dc.citation.endPage57-
dc.citation.startPage51-
dc.citation.volume30-
dc.contributor.affiliatedAuthorSeungmo Lim-
dc.contributor.affiliatedAuthorJae Sun Moon-
dc.contributor.alternativeName남문-
dc.contributor.alternativeName김정선-
dc.contributor.alternativeName임승모-
dc.contributor.alternativeName박충열-
dc.contributor.alternativeName김정규-
dc.contributor.alternativeName최홍수-
dc.contributor.alternativeName임현섭-
dc.contributor.alternativeName문제선-
dc.contributor.alternativeName이수헌-
dc.identifier.bibliographicCitationPlant Pathology Journal, vol. 30, no. 1, pp. 51-57-
dc.identifier.doi10.5423/PPJ.OA.08.2013.0084-
dc.subject.keywordLarge-scale oligonucleotide chip-
dc.subject.keywordLSON chip-
dc.subject.keywordOligo chip-
dc.subject.keywordPlant virus-
dc.subject.keywordVirus diagnosis-
dc.subject.localLarge-scale oligonucleotide chip-
dc.subject.localLSON chip-
dc.subject.localOligo chip-
dc.subject.localPlant virus-
dc.subject.localPlant viruses-
dc.subject.localplant virus-
dc.subject.localVirus diagnosis-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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