Selection of aptamers for mature white adipocytes by cell SELEX using flow cytometry

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dc.contributor.authorEun Young Kim-
dc.contributor.authorJi Won Kim-
dc.contributor.authorWon Kon Kim-
dc.contributor.authorBaek Soo Han-
dc.contributor.authorSung Goo Park-
dc.contributor.authorBong Hyun Chung-
dc.contributor.authorSang Chul Lee-
dc.contributor.authorKwang-Hee Bae-
dc.date.accessioned2017-04-19T09:53:12Z-
dc.date.available2017-04-19T09:53:12Z-
dc.date.issued2014-
dc.identifier.issn1932-6203-
dc.identifier.uri10.1371/journal.pone.0097747ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11988-
dc.description.abstractBackground: Adipose tissue, mainly composed of adipocytes, plays an important role in metabolism by regulating energy homeostasis. Obesity is primarily caused by an abundance of adipose tissue. Therefore, specific targeting of adipose tissue is critical during the treatment of obesity, and plays a major role in overcoming it. However, the knowledge of cell-surface markers specific to adipocytes is limited. Methods and Results: We applied the CELL SELEX (Systematic Evolution of Ligands by EXponential enrichment) method using flow cytometry to isolate molecular probes for specific recognition of adipocytes. The aptamer library, a mixture of FITC-tagged single-stranded random DNAs, is used as a source for acquiring molecular probes. With the increasing number of selection cycles, there was a steady increase in the fluorescence intensity toward mature adipocytes. Through 12 rounds of SELEX, enriched aptamers showing specific recognition toward mature 3T3-L1 adipocyte cells were isolated. Among these, two aptamers (MA-33 and 91) were able to selectively bind to mature adipocytes with an equilibrium dissociation constant (Kd) in the nanomolar range. These aptamers did not bind to preadipocytes or other cell lines (such as HeLa, HEK-293, or C2C12 cells). Additionally, it was confirmed that MA-33 and 91 can distinguish between mature primary white and primary brown adipocytes. Conclusions: These selected aptamers have the potential to be applied as markers for detecting mature white adipocytes and monitoring adipogenesis, and could emerge as an important tool in the treatment of obesity.-
dc.publisherPublic Library of Science-
dc.titleSelection of aptamers for mature white adipocytes by cell SELEX using flow cytometry-
dc.title.alternativeSelection of aptamers for mature white adipocytes by cell SELEX using flow cytometry-
dc.typeArticle-
dc.citation.titlePLoS One-
dc.citation.number5-
dc.citation.endPagee97747-
dc.citation.startPagee97747-
dc.citation.volume9-
dc.contributor.affiliatedAuthorEun Young Kim-
dc.contributor.affiliatedAuthorJi Won Kim-
dc.contributor.affiliatedAuthorWon Kon Kim-
dc.contributor.affiliatedAuthorBaek Soo Han-
dc.contributor.affiliatedAuthorSung Goo Park-
dc.contributor.affiliatedAuthorBong Hyun Chung-
dc.contributor.affiliatedAuthorSang Chul Lee-
dc.contributor.affiliatedAuthorKwang-Hee Bae-
dc.contributor.alternativeName김은영-
dc.contributor.alternativeName김지원-
dc.contributor.alternativeName김원곤-
dc.contributor.alternativeName한백수-
dc.contributor.alternativeName박성구-
dc.contributor.alternativeName정봉현-
dc.contributor.alternativeName이상철-
dc.contributor.alternativeName배광희-
dc.identifier.bibliographicCitationPLoS One, vol. 9, no. 5, pp. e97747-e97747-
dc.identifier.doi10.1371/journal.pone.0097747-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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