Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

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dc.contributor.authorHyun A Kim-
dc.contributor.authorSuk Yoon Kwon-
dc.contributor.authorM S Yang-
dc.contributor.authorP S Choi-
dc.date.accessioned2017-04-19T09:53:13Z-
dc.date.available2017-04-19T09:53:13Z-
dc.date.issued2014-
dc.identifier.issn12292818-
dc.identifier.uri10.5010/JPB.2014.41.1.50ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/11990-
dc.description.abstractPlant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.-
dc.publisherSouth Korea-
dc.titleExpression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate-
dc.title.alternativeExpression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate-
dc.typeArticle-
dc.citation.titleJournal of Plant Biotechnology-
dc.citation.number1-
dc.citation.endPage55-
dc.citation.startPage50-
dc.citation.volume41-
dc.contributor.affiliatedAuthorSuk Yoon Kwon-
dc.contributor.alternativeName김현아-
dc.contributor.alternativeName권석윤-
dc.contributor.alternativeName양문식-
dc.contributor.alternativeName최필선-
dc.identifier.bibliographicCitationJournal of Plant Biotechnology, vol. 41, no. 1, pp. 50-55-
dc.identifier.doi10.5010/JPB.2014.41.1.50-
dc.subject.keywordDengue virus-
dc.subject.keywordEdible vaccine-
dc.subject.keywordImmature embryo-
dc.subject.keywordMaize transformation-
dc.subject.keywordParomomycin-
dc.subject.localDengue virus-
dc.subject.localEdible vaccine-
dc.subject.localImmature embryo-
dc.subject.localMaize transformation-
dc.subject.localParomomycin-
dc.description.journalClassN-
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Division of Biomaterials Research > 1. Journal Articles
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