Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathway = Methoxychlor와 triclosan에 의한 난소암 성장촉진은 세포주기와 아포토시스 관련 유전자

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Title
Methoxychlor and triclosan stimulates ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an estrogen receptor-dependent pathway = Methoxychlor와 triclosan에 의한 난소암 성장촉진은 세포주기와 아포토시스 관련 유전자
Author(s)
J Y Kim; B R Yi; R E Go; K A Hwang; Ki Hoan Nam; K C Choi
Bibliographic Citation
Environmental Toxicology and Pharmacology, vol. 37, no. 3, pp. 1264-1274
Publication Year
2014
Abstract
Methoxychlor and triclosan are emergent or suspected endocrine-disrupting chemicals (EDCs). Methoxychlor [MXC; 1,1,1-trichlor-2,2-bis (4-methoxyphenyl) ethane] is an organochlorine pesticide that has been primarily used since dichlorodiphenyltrichloroethane (DDT) was banned. In addition, triclosan (TCS) is used as a common component of soaps, deodorants, toothpastes, and other hygiene products at concentrations up to 0.3%. In the present study, the potential impact of MXC and TCS on ovarian cancer cell growth and underlying mechanism(s) was examined following their treatments in BG-1 ovarian cancer cells. As results, MXC and TCS induced BG-1 cell growth via regulating cyclin D1, p21 and Bax genes related with cell cycle and apoptosis. A methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay confirmed that the proliferation of BG-1 ovarian cancer cells was stimulated by MXC (10-6, 10-7, 10-8, and 10-9M) or TCS (10-6, 10-7, 10-8, and 10-9M). Treatment of BG-1 cells with MXC or TCS resulted in the upregulation of cyclin D1 and downregulation of p21 and Bax transcriptions. In addition, the protein level of cyclin D1 was increased by MXC or TCS while p21 and Bax protein levels appeared to be reduced in these cells. Furthermore, MXC- or TCS-induced alterations of these genes were reversed in the presence of ICI 182,780 (10-7M), suggesting that the changes in these gene expressions may be regulated by an ER-dependent signaling pathway. In conclusion, the results of our investigation indicate that two potential EDCs, MXC and TCS, may stimulate ovarian cancer growth by regulating cell cycle- and apoptosis-related genes via an ER-dependent pathway.
Keyword
Endocrine-disrupting chemicalsEstrogen receptorsMethoxychlorOvarian cancerTriclosan
ISSN
1382-6689
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.etap.2014.04.013
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of National Bio-Infrastructure > Laboratory Animal Resource & Research Center > 1. Journal Articles
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