Relative quantification in imaging of a peptide on a mouse brain tissue by matrix-assisted laser desorption ionization

Cited 15 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorK M Park-
dc.contributor.authorJeong Hee Moon-
dc.contributor.authorK P Kim-
dc.contributor.authorS H Lee-
dc.contributor.authorM S Kim-
dc.date.accessioned2017-04-19T09:54:00Z-
dc.date.available2017-04-19T09:54:00Z-
dc.date.issued2014-
dc.identifier.issn00032700-
dc.identifier.uri10.1021/ac500911xko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12021-
dc.description.abstractIt is well-known that the number of analyte ions generated by matrix-assisted laser desorption ionization (MALDI) is not directly proportional to the analyte concentration at the irradiated spot. This is an obstacle to acquiring quantitatively meaningful maps for materials in a tissue by MALDI imaging. The problem worsens as the matrix suppression due to contaminants in the sample increases. In this work, we use a peptide as an example and show that we can overcome this problem by utilizing three guidelines derived from our recent studies on the generation of reproducible MALDI spectra. First is to acquire MALDI spectra under a temperature-controlled condition. Second is to keep the matrix suppression below an experimentally determined limit, and the third is to construct the image map using the peptide-to-matrix ion abundance ratio rather than the peptide ion abundance. The strategy works well for contaminated tissue samples and generates quantitatively meaningful maps. Also, it is demonstrated that a preposterous map can be generated when the peptide ion abundance is used in the construction of the map.-
dc.publisherAmer Chem Soc-
dc.titleRelative quantification in imaging of a peptide on a mouse brain tissue by matrix-assisted laser desorption ionization-
dc.title.alternativeRelative quantification in imaging of a peptide on a mouse brain tissue by matrix-assisted laser desorption ionization-
dc.typeArticle-
dc.citation.titleAnalytical Chemistry-
dc.citation.number10-
dc.citation.endPage5135-
dc.citation.startPage5131-
dc.citation.volume86-
dc.contributor.affiliatedAuthorJeong Hee Moon-
dc.contributor.alternativeName박경-
dc.contributor.alternativeName문정희-
dc.contributor.alternativeName김광-
dc.contributor.alternativeName이성-
dc.contributor.alternativeName김명수-
dc.identifier.bibliographicCitationAnalytical Chemistry, vol. 86, no. 10, pp. 5131-5135-
dc.identifier.doi10.1021/ac500911x-
dc.description.journalClassY-
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.