Label-free measurement of cell viability via counting cells attached on affinity substrates

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Title
Label-free measurement of cell viability via counting cells attached on affinity substrates
Author(s)
Junhyoung Ahn; Jina Park; Yeon-Gu KimEun Gyo Lee; M G Kim; Yong Beom Shin
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 19, no. 2, pp. 257-261
Publication Year
2014
Abstract
The commonly used trypan blue dye exclusion method and other modified cell viability methods, such as fluorescein dye and tetrazolium dye exclusion, artificially introduce toxic chemicals to cells and, thus, alter cellular organelles when measuring cell viability. Therefore, cell viability could be affected by the processes currently used to observe viability. In this study, the cell viability of Chinese hamster ovary (CHO) cells was measured by simply counting attached cells after the cultured CHO cells were attached on a Concanavalin A (Con A) substrate. The efficiency of cell attachment to Con A surfaces was different for live and dead cells allowing the cell viability of CHO cells to be measured without any chemical modifications to the cells.
Keyword
label-free measurementaffinity bindingcell attachmentcell viabilityCHO cell
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12257-013-0556-1
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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