Application of internal transcribed spacer of nuclear ribosomal DNA for identification of Echinops mandavillei Kit Tan

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Title
Application of internal transcribed spacer of nuclear ribosomal DNA for identification of Echinops mandavillei Kit Tan
Author(s)
F M A Al-Hemaid; M A Ali; Joongku Lee; G Gyulai; A K Pandey
Bibliographic Citation
Bangladesh Journal of Plant Taxonomy, vol. 21, no. 1, pp. 33-42
Publication Year
2014
Abstract
The present study explored the use of internal transcribed spacers (ITS) sequences (ITS1-5.8S-ITS2) of nuclear ribosomal DNA (nrDNA) for identification of Echinops mandavillei Kit Tan, an endemic species to Saudi Arabia. The sequence similarity search using Basic Local Alignment Search Tool (BLAST) and phylogenetic analyses of the ITS sequence of E. mandavillei Kit Tan showed high level of sequence similarity (98%) with E. glaberrimus DC. (section Ritropsis). The novel primary sequence and the secondary structure of ITS2 of E. mandavillei could have a potential use for molecular genotyping.
Keyword
AsteraceaeEchinops mandavilleiEndemicITSnrDNASaudi arabia
ISSN
1028-2092
Publisher
Bangladesh Assoc Plant Taxonomists
DOI
http://dx.doi.org/10.3329/bjpt.v21i1.19256
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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