Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system

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Title
Misidentification of Candida guilliermondii as C. famata among strains isolated from blood cultures by the VITEK 2 system
Author(s)
S H Kim; J H Shin; J H Mok; S Y Kim; S A Song; H R Kim; J K Kook; Young Hyo Chang; I K Bae; K Lee
Bibliographic Citation
Biomed Research International, vol. 2014, pp. 250408-250408
Publication Year
2014
Abstract
Introduction. The aim of this study was to differentiate between Candida famata and Candida guilliermondii correctly by using matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and gene sequencing. Methods. Twenty-eight Candida strains from blood cultures that had been identified as C. famata (N=25), C. famata/C. guilliermondii (N=2), and C. guilliermondii (N=1) by the VITEK 2 system using the YST ID card were included. We identified these strains by MALDI-TOF MS and gene sequencing using the 28S rRNA and ITS genes and compared the results with those obtained by the VITEK 2 system. Results. All 28 isolates were finally identified as C. guilliermondii. Sequencing analysis of the 28S rRNA gene showed 99.80%-100% similarity with C. guilliermondii for all 28 strains. The ITS gene sequencing of the strains showed 98.34%-100% homology with C. guilliermondii. By MALDI-TOF, we could correctly identify 21 (75%) of 28 C. guilliermondii isolates. Conclusion. We should suspect misidentification when C. famata is reported by the VITEK 2 system, and we always should keep in mind the possibility of misidentification of any organism when an uncommon species is reported.
ISSN
2314-6133
Publisher
Hindawi Ltd
DOI
http://dx.doi.org/10.1155/2014/250408
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > ABS Research Support Center > 1. Journal Articles
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