Pseudoruegeria limi sp. nov. isolated from mud flats in the Yellow Sea in Korea

Abstract

A Gram-negative, aerobic, non-motile and rod-shaped bacterial strain, D-17T, was isolated from mud flats in the Yellow Sea in Korea. Phylogenetic trees based on the 16S rRNA gene sequence showed that strain D-17T belongs to the genus Pseudoruegeria and it shared 97.5 % similarity with the type strain of Pseudoruegeria haliotis WM67T. The sequence similarities with Pseudoruegeria litimaris HD-43T and Pseudoruegeria aquimaris SW-255T were 96.9 and 96.1 %, respectively. Strain D-17T was found to grow with 0.5-6 % (w/v) NaCl, at 20-30°C, and at pH 6.5-8.0. Strain D-17T was determined to contain Q-10 as the predominant ubiquinone and summed feature 8 (C 18:1 ω7c and/or C18:1 ω6c, as defined by the MIDI system) as the major fatty acids. The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, an unidentified aminolipid, an unidentified glycolipid, an unidentified lipid and four unidentified phospholipids. The DNA G+C content was determined to be 63.6 mol%. The DNA-DNA relatedness with P. haliotis WM67T was 32.5 %. The differential phenotypic properties revealed that strain D-17T can be separated from other Pseudoruegeria species. Based on the data presented in this study, strain D-17T represents a novel species, for which the name Pseudoruegeria limi sp. nov. is proposed. The type strain is D-17T (=KCTC 32460T =JCM 19487T).