Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress

Cited 55 time in scopus
Metadata Downloads
Title
Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress
Author(s)
Seung-Bin Yoon; Seon-A Choi; Bo Woong SimJi-Su Kim; Seong-Eun Mun; Pil-Soo Jeong; Hae-Jun Yang; Youngjeon LeeYoung-Ho ParkBong Seok SongYoung-Hyun KimKang Jin JeongJae Won Huh; Sang-Rae Lee; Sun-Uk Kim; Kyu Tae Chang
Bibliographic Citation
Biology of Reproduction, vol. 90, no. 5, pp. 104-113
Publication Year
2014
Abstract
The stress produced by the coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) has been explored extensively, but little is known regarding their roles in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared with the tension produced by 5% O2, 20% O2 significantly decreased the blastocyst formation rate and cell survival, which was accompanied by increases in ROS and in levels of sXBP-1 transcript, which is an ER stress indicator. In addition, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment in developing bovine embryos. Consistent with this observation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst developmental rate, trophectoderm proportion, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation with TUDCA, suggesting a possible mechanism governing the mutual regulation between ROS and ER stress. Interestingly, knockdown of XBP-1 transcripts resulted in both elevation of ROS and decrease of antioxidant transcripts, which ultimately reduced in vitro developmental competence of bovine embryos. Based on these results, in vitro developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stresses. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.
Keyword
early developmentembryo cultureendoplasmic reticulum stressreactive oxygen species
ISSN
0006-3363
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1095/biolreprod.113.113480
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.