Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress
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- Developmental competence of bovine early embryos depends on the coupled response between oxidative and endoplasmic reticulum stress
- Seung-Bin Yoon; Seon-A Choi; Bo Woong Sim; Ji-Su Kim; Seong-Eun Mun; Pil-Soo Jeong; Hae-Jun Yang; Youngjeon Lee; Young-Ho Park; Bong Seok Song; Young-Hyun Kim; Kang Jin Jeong; Jae Won Huh; Sang-Rae Lee; Sun-Uk Kim; Kyu Tae Chang
- Bibliographic Citation
- Biology of Reproduction, vol. 90, no. 5, pp. 104-113
- Publication Year
- The stress produced by the coupling of reactive oxygen species (ROS) and endoplasmic reticulum (ER) has been explored extensively, but little is known regarding their roles in the early development of mammalian embryos. Here, we demonstrated that the early development of in vitro-produced (IVP) bovine embryos was governed by the cooperative action between ROS and ER stress. Compared with the tension produced by 5% O2, 20% O2 significantly decreased the blastocyst formation rate and cell survival, which was accompanied by increases in ROS and in levels of sXBP-1 transcript, which is an ER stress indicator. In addition, treatment with glutathione (GSH), a ROS scavenger, decreased ROS levels, which resulted in increased blastocyst formation and cell survival rates. Importantly, levels of sXBP-1 and ER stress-associated transcripts were reduced by GSH treatment in developing bovine embryos. Consistent with this observation, tauroursodeoxycholate (TUDCA), an ER stress inhibitor, improved blastocyst developmental rate, trophectoderm proportion, and cell survival. Moreover, ROS and sXBP-1 transcript levels were markedly decreased by supplementation with TUDCA, suggesting a possible mechanism governing the mutual regulation between ROS and ER stress. Interestingly, knockdown of XBP-1 transcripts resulted in both elevation of ROS and decrease of antioxidant transcripts, which ultimately reduced in vitro developmental competence of bovine embryos. Based on these results, in vitro developmental competence of IVP bovine embryos was highly dependent on the coupled response between oxidative and ER stresses. These results increase our understanding of the mechanism(s) governing early embryonic development and may improve strategies for the generation of IVP embryos with high developmental competence.
- early development; embryo culture; endoplasmic reticulum stress; reactive oxygen species
- Oxford Univ Press
- Appears in Collections:
- Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
Ochang Branch Institute > Division of Bioinfrastructure > National Primate Research Center > 1. Journal Articles
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