Fermentation process for mass production of clitocybin A, a new anti-wrinkle agent from Clitocybe aurantiaca and evaluation of inhibitory activity on matrix metalloproteinase-1 expression=Clitocybe aurantiaca균주의 주름개선소재 clitocybin A의 생산과 MMP-1

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dc.contributor.authorK C Kim-
dc.contributor.authorHyeok Won Lee-
dc.contributor.authorHong-Weon Lee-
dc.contributor.authorSoo Jin Choo-
dc.contributor.authorIck-Dong Yoo-
dc.contributor.authorB J Ha-
dc.date.accessioned2017-04-19T09:54:44Z-
dc.date.available2017-04-19T09:54:44Z-
dc.date.issued2014-
dc.identifier.issn0257-2389-
dc.identifier.uri10.4014/kjmb.1405.05002ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12060-
dc.description.abstractClitocybin A is a novel anti-wrinkle cosmetic agent produced by the strain from a Korean native mushroom Clitocybe aurantiaca. In this study, fermentation, extraction, and purification conditions for a large scale production of clitocybin A were optimized, and its cytotoxicity and inhibition activity on the expression of matrix metalloproteinase-1 (MMP-1) were characterized. The mass production of anti-wrinkle agent was achieved according to the 300 L fermentation process with a fedbatch cultivation using the modified yeast-maltose (YM) broth, and a total of 12.5 kg of cell mass was obtained in a 120 L culture broth for 14 days. After extraction and purification, clitocybin A was identified by HPLC. The cytotoxicity of clitocybin A was examined by the MTT assay. When assayed at 100 and 200 μg/ml concentrations, clitocybin A showed no cytotoxicity, demonstrating safety. The inhibition activity of clitocybin A on the expression of MMP-1 was examined against UV irradiation. Oleanolic acid (control group) showed a relatively low MMP-1 inhibiting activity (ca. 16.7%) at 10 μg/ml and showed increased cytotoxicity at higher concentrations. In contrast, clitocybin A showed no cytotoxicity at 100 μg/ml, and exhibited a relatively high MMP-1-inhibiting activity (33.1%). These findings indicate that clitocybin A may be a safe and effective anti-wrinkle agent for use in functional cosmetics.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleFermentation process for mass production of clitocybin A, a new anti-wrinkle agent from Clitocybe aurantiaca and evaluation of inhibitory activity on matrix metalloproteinase-1 expression=Clitocybe aurantiaca균주의 주름개선소재 clitocybin A의 생산과 MMP-1-
dc.title.alternativeFermentation process for mass production of clitocybin A, a new anti-wrinkle agent from Clitocybe aurantiaca and evaluation of inhibitory activity on matrix metalloproteinase-1 expression-
dc.typeArticle-
dc.citation.titleKorean Journal of (Applied) Microbiology & Biotechnology-
dc.citation.number2-
dc.citation.endPage201-
dc.citation.startPage194-
dc.citation.volume42-
dc.contributor.affiliatedAuthorHyeok Won Lee-
dc.contributor.affiliatedAuthorHong-Weon Lee-
dc.contributor.affiliatedAuthorSoo Jin Choo-
dc.contributor.affiliatedAuthorIck-Dong Yoo-
dc.contributor.alternativeName김관철-
dc.contributor.alternativeName이혁원-
dc.contributor.alternativeName이홍원-
dc.contributor.alternativeName추수진-
dc.contributor.alternativeName유익동-
dc.contributor.alternativeName하병조-
dc.identifier.bibliographicCitationKorean Journal of (Applied) Microbiology & Biotechnology, vol. 42, no. 2, pp. 194-201-
dc.identifier.doi10.4014/kjmb.1405.05002-
dc.subject.keywordAnti-aging-
dc.subject.keywordAnti-wrinkle-
dc.subject.keywordClitocybe aurantiaca-
dc.subject.keywordClitocybin A-
dc.subject.keywordMMP-1-
dc.subject.localanti-aging-
dc.subject.localAnti-aging-
dc.subject.localanti-wrinkle-
dc.subject.localAntiwrinkle-
dc.subject.localAnti-wrinkle-
dc.subject.localclitocybe aurantiaca-
dc.subject.localClitocybe aurantiaca-
dc.subject.localClitocybin A-
dc.subject.localclitocybin A-
dc.subject.localMMP-1-
dc.description.journalClassN-
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Bio Technology Innovation > 1. Journal Articles
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