Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system

Cited 35 time in scopus
Metadata Downloads

Full metadata record

DC FieldValueLanguage
dc.contributor.authorN Lee-
dc.contributor.authorJ W Park-
dc.contributor.authorH J Kim-
dc.contributor.authorJ H Yeon-
dc.contributor.authorJ Kwon-
dc.contributor.authorJ J Ko-
dc.contributor.authorS H Oh-
dc.contributor.authorH S Kim-
dc.contributor.authorA Kim-
dc.contributor.authorBaek Soo Han-
dc.contributor.authorSang Chul Lee-
dc.contributor.authorN L Jeon-
dc.contributor.authorJ Song-
dc.date.accessioned2017-04-19T09:55:16Z-
dc.date.available2017-04-19T09:55:16Z-
dc.date.issued2014-
dc.identifier.issn1016-8478-
dc.identifier.uri10.14348/molcells.2014.0137ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/12095-
dc.description.abstractMicrofluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.-
dc.publisherKorea Soc-Assoc-Inst-
dc.titleMonitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system-
dc.title.alternativeMonitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system-
dc.typeArticle-
dc.citation.titleMolecules and Cells-
dc.citation.number6-
dc.citation.endPage502-
dc.citation.startPage497-
dc.citation.volume37-
dc.contributor.affiliatedAuthorBaek Soo Han-
dc.contributor.affiliatedAuthorSang Chul Lee-
dc.contributor.alternativeName이나연-
dc.contributor.alternativeName박재우-
dc.contributor.alternativeName김형준-
dc.contributor.alternativeName연주훈-
dc.contributor.alternativeName권지혜-
dc.contributor.alternativeName고정재-
dc.contributor.alternativeName오승훈-
dc.contributor.alternativeName김현숙-
dc.contributor.alternativeName김애리-
dc.contributor.alternativeName한백수-
dc.contributor.alternativeName이상철-
dc.contributor.alternativeName전누리-
dc.contributor.alternativeName송지환-
dc.identifier.bibliographicCitationMolecules and Cells, vol. 37, no. 6, pp. 497-502-
dc.identifier.doi10.14348/molcells.2014.0137-
dc.subject.keywordAxons-
dc.subject.keywordHuman embryonic stem cells-
dc.subject.keywordMicrofluidics-
dc.subject.keywordMigration-
dc.subject.keywordNeural differentiation-
dc.subject.localAxons-
dc.subject.localHuman embryonic stem cells-
dc.subject.localHuman embryonic stem cell-
dc.subject.localHuman embryonic stem cells (hESCs)-
dc.subject.localHuman Embryonic Stem cell-
dc.subject.localhuman embryonic stem cell-
dc.subject.localmicrofluidics-
dc.subject.localMicrofluidics-
dc.subject.localMigration-
dc.subject.localmigration-
dc.subject.localNeural differentiation-
dc.description.journalClassY-
Appears in Collections:
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.