DC Field | Value | Language |
---|---|---|
dc.contributor.author | N Lee | - |
dc.contributor.author | J W Park | - |
dc.contributor.author | H J Kim | - |
dc.contributor.author | J H Yeon | - |
dc.contributor.author | J Kwon | - |
dc.contributor.author | J J Ko | - |
dc.contributor.author | S H Oh | - |
dc.contributor.author | H S Kim | - |
dc.contributor.author | A Kim | - |
dc.contributor.author | Baek Soo Han | - |
dc.contributor.author | Sang Chul Lee | - |
dc.contributor.author | N L Jeon | - |
dc.contributor.author | J Song | - |
dc.date.accessioned | 2017-04-19T09:55:16Z | - |
dc.date.available | 2017-04-19T09:55:16Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | 10.14348/molcells.2014.0137 | ko |
dc.identifier.uri | https://oak.kribb.re.kr/handle/201005/12095 | - |
dc.description.abstract | Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells. | - |
dc.publisher | Korea Soc-Assoc-Inst | - |
dc.title | Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system | - |
dc.title.alternative | Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system | - |
dc.type | Article | - |
dc.citation.title | Molecules and Cells | - |
dc.citation.number | 6 | - |
dc.citation.endPage | 502 | - |
dc.citation.startPage | 497 | - |
dc.citation.volume | 37 | - |
dc.contributor.affiliatedAuthor | Baek Soo Han | - |
dc.contributor.affiliatedAuthor | Sang Chul Lee | - |
dc.contributor.alternativeName | 이나연 | - |
dc.contributor.alternativeName | 박재우 | - |
dc.contributor.alternativeName | 김형준 | - |
dc.contributor.alternativeName | 연주훈 | - |
dc.contributor.alternativeName | 권지혜 | - |
dc.contributor.alternativeName | 고정재 | - |
dc.contributor.alternativeName | 오승훈 | - |
dc.contributor.alternativeName | 김현숙 | - |
dc.contributor.alternativeName | 김애리 | - |
dc.contributor.alternativeName | 한백수 | - |
dc.contributor.alternativeName | 이상철 | - |
dc.contributor.alternativeName | 전누리 | - |
dc.contributor.alternativeName | 송지환 | - |
dc.identifier.bibliographicCitation | Molecules and Cells, vol. 37, no. 6, pp. 497-502 | - |
dc.identifier.doi | 10.14348/molcells.2014.0137 | - |
dc.subject.keyword | Axons | - |
dc.subject.keyword | Human embryonic stem cells | - |
dc.subject.keyword | Microfluidics | - |
dc.subject.keyword | Migration | - |
dc.subject.keyword | Neural differentiation | - |
dc.subject.local | Axons | - |
dc.subject.local | Human embryonic stem cells | - |
dc.subject.local | Human embryonic stem cell | - |
dc.subject.local | Human embryonic stem cells (hESCs) | - |
dc.subject.local | Human Embryonic Stem cell | - |
dc.subject.local | human embryonic stem cell | - |
dc.subject.local | microfluidics | - |
dc.subject.local | Microfluidics | - |
dc.subject.local | Migration | - |
dc.subject.local | migration | - |
dc.subject.local | Neural differentiation | - |
dc.description.journalClass | Y | - |
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