Efficient secretory expression of recombinant endoxylanase from Bacillus sp. HY-20 in Saccharomyces cerevisiae = 출아효모에서 Bacillus sp. HY-20 균주의 재조합 endoxylanse의 효율적 분비 발현

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Title
Efficient secretory expression of recombinant endoxylanase from Bacillus sp. HY-20 in Saccharomyces cerevisiae = 출아효모에서 Bacillus sp. HY-20 균주의 재조합 endoxylanse의 효율적 분비 발현
Author(s)
M J Kim; B H Kim; S W Nam; Eui Sung Choi; D H Shin; Han Young Cho; Kwang-Hee SonHo Yong Park; Y H Kim
Bibliographic Citation
Journal of Life Science, vol. 23, no. 7, pp. 863-868
Publication Year
2013
Abstract
The XylP gene, which encodes endoxylanase in Bacillus sp. HY-20, was subcloned, and two expression plasmids, pG-xylP and pGMF-xylP were constructed. These plasmids, which contain different signal sequences, XylP s.s and MFαopt s.s, respectively, for the secretory expression of endoxylanase, were transformed into Saccharomyces cerevisiae SEY2102 and FY833, respectively. The recombinant endoxylanases were successfully expressed, with a total activity range of 23.7?70.1 unit/ml according to the expression system and host strain. The endoxylanase activity in SEY2102/pGMF-xylP reached a maximum of 88.1 unit/ml in baffled flask culture. Most of the recombinant endoxylanase was efficiently secreted in the extracellular fraction, and the MFαopt s.s was more efficient for secreting endoxylanase in yeast than the XylP s.s. Therefore, the expression system developed in this study produces large extracellular amounts of endoxylanase using S. cerevisiae as the host strain, and it could be used in bioethanol production and industrial applications.
Keyword
Endoxylanaseoptimized MFα signal sequence (MFαopt s.s)GAL promotersecretory productionSaccharomyces cerevisiae
ISSN
I000-0128
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2013.23.7.863
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
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