Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high-level production of L-2,3-butanediol

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Title
Identification and characterization of a short-chain acyl dehydrogenase from Klebsiella pneumoniae and its application for high-level production of L-2,3-butanediol
Author(s)
Jang Min Park; Won-Kyung Hong; Sung Mok Lee; Sun-Yeon Heo; You Ree Jung; In Yeong Kang; Baek Rock OhJeong-Woo SeoChul Ho Kim
Bibliographic Citation
Journal of Industrial Microbiology & Biotechnology, vol. 41, no. 9, pp. 1425-1433
Publication Year
2014
Abstract
Klebsiella pneumoniae synthesize large amounts of L-2,3-butanediol (L-2,3-BD), but the underlying mechanism has been unknown. In this study, we provide the first identification and characterization of an L-2,3-BD dehydrogenase from K. pneumoniae, demonstrating its reductive activities toward diacetyl and acetoin, and oxidative activity toward L-2,3-BD. Optimum pH, temperature, and kinetics determined for reductive and oxidative reactions support the preferential production of 2,3-BD during cell growth. Synthesis of L-2,3-BD was remarkably enhanced by increasing gene dosage, reaching levels that, to the best of our knowledge, are the highest achieved to date.
Keyword
2,3-Butanediol dehydrogenase/acetoin reductaseKlebsiella pneumoniaL-2,3-butanediolStereospecificity
ISSN
0169-4146
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10295-014-1483-7
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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